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Plasmid#65524PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
HNRNPCL1_RRM_pGEX
Plasmid#135116PurposeRecombinant expression of HNRNPCL1 with dual-affinity tagDepositorInsertHNRNPCL1 (HNRNPCL1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RBM15B_3xRRM_pGEX
Plasmid#135125PurposeRecombinant expression of RBM15B with dual-affinity tagDepositorInsertRBM15B (RBM15B Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-RPG
Plasmid#85932PurposeConstitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cellsDepositorInsertpPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
UseDual-reporter surrogateTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC5
Plasmid#183200PurposeEntry cloneDepositorInsertCas9-RFC5
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE652
Plasmid#153230PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE342
Plasmid#153251PurposeM8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBT-R16
Plasmid#31827DepositorInsertmini Tol2 ITR
UseCre/Lox; Gene trapTagsmRFPExpressionMutationmRFP lacks start codonPromoterbeta ActinAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDGE671
Plasmid#153237PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX1_2xChromo
Plasmid#179393PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromoDepositorInsertCBX1_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-pCdc42
Plasmid#23240DepositorInsertCdc42 (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-bCdc42
Plasmid#23241DepositorInsertCdc42 (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-hDcp2
Plasmid#72213Purposemammalian expression of Flag-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsFlagExpressionMammalianMutationQ170R (please see depositor's comment below)PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GTH-ir
Plasmid#81107PurposeGene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationPromoterAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAF15_RRM-ZNF_pGEX
Plasmid#135111PurposeRecombinant expression of TAF15 with dual-affinity tagDepositorInsertTAF15 (TAF15 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9 GW STOP
Plasmid#183196PurposeDestination vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBFOX3_RRM_pGEX
Plasmid#135131PurposeRecombinant expression of RBFOX3 with dual-affinity tagDepositorInsertRBFOX3 (RBFOX3 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only