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Plasmid#172709PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA2
Plasmid#172710PurposeCas9 with 3' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OptoPB
Plasmid#113935PurposeExpress a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactionsDepositorInsertOptoPB
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
plko.1/shMCT4 #474
Plasmid#99597PurposeKnockdown of MCT4 expressionDepositorInsertMCT4 (SLC16A3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
piGECI-NES-N1
Plasmid#160422PurposeExpression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequenceDepositorInsertiGECI-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtPT4Pro
Plasmid#159414PurposeMedicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtPT4 Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtBCP1Pro
Plasmid#159415PurposeMedicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtBCP1 Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL2
Plasmid#48742PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +386)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-UAG site)
Plasmid#170138PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(UAG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
plko.1/shMCT4 477
Plasmid#99598PurposeKnockdown of MCT4 expressionDepositorInsertMCT4 (SLC16A3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4
Plasmid#48744PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1536 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +1536)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-3
Plasmid#193696PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MCV-Rep-
Plasmid#32058DepositorInsertMCV-Rep- genome
UseTagsExpressionMutationSingle cytosine to adenine (C/A) mutation at 2,07…Promoternone from vector. Insert contains native promoter…Available sinceOct. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GSX2-tTA-BleoR
Plasmid#161748PurposeTet-ON tTA plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertAdvanced tTA
UseCRISPRTagsExpressionMammalianMutationPromoterendogenous GSX2 promoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)
Plasmid#87735PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AQTFAATHNPWASQA_epitope
Plasmid#165019PurposeExpression of CMV UL86 (1222-1236) fused to CD74DepositorInsertCMV UL86 (1222-1236)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL1
Plasmid#48741PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to -141)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only