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Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3b
Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3a
Plasmid#166053Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspaseDepositorInsertOrbicella faveolata caspase-3a
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp3
Plasmid#166052Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertPorites astreiodes caspase-3
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp7a
Plasmid#166051Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspaseDepositorInsertPorites astreiodes caspase-7a
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbH-C-A2-PCP S1524A
Plasmid#114158PurposeExpresses ClbH-C-A2-PCP S1524ADepositorInsertclbH
UseTagsS-Tag, Thrombin siteExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbH with S…PromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mEGFP
Plasmid#105143PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mEGFP
UseTags81nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas2B
Plasmid#82373PurposedCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas45
Plasmid#82390PurposesgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas7
Plasmid#82379PurposedCas9 expressed from low constituve promoter, c225, in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoterc225Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2E
Plasmid#82376PurposedCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2A
Plasmid#82372PurposedCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas39
Plasmid#82389PurposesgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas36
Plasmid#82388PurposesgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas35
Plasmid#82387PurposesgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas28
Plasmid#82385PurposesgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23108Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas27
Plasmid#82384PurposesgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23117Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas15
Plasmid#82383PurposesgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas14
Plasmid#82382PurposesgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only