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Plasmid#117499PurposeFAST-Red selectable marker Golden Gate Level 1 Position 1DepositorInsertBpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
UseTagsRFPExpressionPlantMutationPromoterAtOLE1Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL50004
Plasmid#50316DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UseTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_7_J23101-RBS34-mCherry-B0015
Plasmid#68141Purposestorage vector for BASIC part: RFP expression cassetteDepositorInsertJ23101-RBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL80007
Plasmid#50323DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL30003
Plasmid#50304DepositorInsertCDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
UseTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL80004
Plasmid#50325DepositorInsertCDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS012
Plasmid#65465PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryDepositorInsertNtag-mCherry
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
JC101
Plasmid#104952PurposeMethanol induing RFP, estrogen inducing GFP, and IPTG inducing CFPDepositorInsertGFP, mCherry, and CFP
UseTagsExpressionMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-P38-che-ssrAec3
Plasmid#27059DepositorInsertP#38-mcherry rfp-ssrAec
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICH49477
Plasmid#50324DepositorInsertCDS, DsRed variant of RFP (Discosoma sp.)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
GPR_control
Plasmid#216222PurposeGFP P2A RFP control reporterDepositorInsertGFP P2A RFP_stop
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
inert_EJC
Plasmid#216224PurposeGFP P2A RFP reporter with inert EJC immediately following stop codonDepositorInsertGFP P2A RFP_inert_EJC
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR_control_lenti
Plasmid#216223PurposeGFP P2A RFP control reporter in a lenti backboneDepositorInsertGFP P2A RFP_stop
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT0
Plasmid#210551PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporterDepositorInsertpU6::AAVS1gRNA::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT1
Plasmid#210552PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporterDepositorInsertpU6::AAVS1gRNA::LT1::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only