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Plasmid#54714PurposeLocalization: Protein Expression Vector , Excitation: 600, Emission: 650DepositorTypeEmpty backboneUseTags6xHis and mNeptuneExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
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pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minTKAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hUBP43
Plasmid#12455DepositorInsertUBP43 (USP18 Human)
UseTagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCFJ906
Plasmid#44472DepositorInsertMinimal Mos1, unc-119, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-AAVS1_sgRNA
Plasmid#183890PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.DepositorInsertAAVS1 sgRNA spacer (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(WT)
Plasmid#84639PurposemCherry-tagged, wild-type LKB1DepositorInsertmCherry-LBK1 (STK11 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_5
Plasmid#60577Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_5
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Merlin S518A
Plasmid#84294PurposeExpression of Merlin with an alanine mutation at the Pak phosphorylation siteDepositorInsertMerlin (NF2) (NF2 Human)
UseTagsEGFPExpressionMammalianMutationS518APromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Merlin S518D
Plasmid#84295PurposeExpression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation siteDepositorInsertMerlin (NF2) (NF2 Human)
UseTagsEGFPExpressionMammalianMutationS518DPromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP443
Plasmid#216361PurposexNup98-Nb1t (+1Cys) Nanobody productionDepositorInsertxNup98-Nb1t (+1Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-TEX264 LIR4A-FLAG
Plasmid#128259PurposeExpresses TEX264 LIR4A in mammalian cellsDepositorInsertTEX264 LIR4A (TEX264 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationChanged FEEL (aa 273-276) to alaninesPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJ1 neo-hu FceRI gamma
Plasmid#16540DepositorInsertFCER1G (FCER1G Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-V96 ELP
Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorInsertGFP fused to ELP (VPGVG) repeated 96 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only