-
Plasmid#186565PurposepMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- tac-YFP
Plasmid#186566PurposepMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CMV/WPREΔU3
Plasmid#190726PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CAG/WPREΔU3
Plasmid#190727PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R160a
Plasmid#173756PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160aDepositorInsertCas9_sgRNA1/R160a_sgRNA2/R160a
UseCRISPRTagsExpressionMutationPromoterAtU6 &StU6Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R160b
Plasmid#173757PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160bDepositorInsertCas9_sgRNA1/R160b_sgRNA2/160b
UseCRISPRTagsExpressionMutationPromoterAtU6 &StU6Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R390a
Plasmid#173758PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390aDepositorInsertCas9_sgRNA1/390a_sgRNA2/390a
UseCRISPRTagsExpressionMutationPromoterAtU6 &StU6Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HseIF1_AC
Plasmid#148441PurposeMammalian Expression of HseIF1DepositorInsertHseIF1 (ELF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF1_AC
Plasmid#148449PurposeMammalian Expression of HseIF1DepositorInsertHseIF1 (ELF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-hCD271-PGK-Neo
Plasmid#186171PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserthCD271
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-EGFP-PGK-Puro
Plasmid#186167PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-Electra1
Plasmid#179476PurposeER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450DepositorInsertCytERM-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA
Plasmid#187064PurposeNpu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Npu
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q
Plasmid#147338PurposeInsect Expression of DmDCP1-GSSGmut-dsRNAresDepositorInsertDmDCP1-GSSGmut-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-AA027
Plasmid#185752PurposeLevel 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).DepositorInsertHygR2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRE-Tn5-142
Plasmid#118531PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-10-mCherry-R11b
Plasmid#179891PurposeDonor with homologous arms for Rab11b to knock in mCherryDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)
Plasmid#179072PurposeVector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membraneDepositorInsertTDH3p-Neon(gfp)-Link-2xPH(PLCd)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)
Plasmid#179038PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membraneDepositorInsertRPL18Bp-Neon(gfp)-Link-2xPH(PLCd)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-EGFP-CTAP-PrP-SBP_W
Plasmid#147835PurposeInsect Expression of EGFPDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
DDX41_p.L237F_p.P238T_EGFP
Plasmid#175845PurposeExpresses DDX41 with mutationDepositorInsertDDX41 (DDX41 Human)
UseTagsEGFPExpressionMammalianMutationc.711G>T, p.L237F and c.712C>A, p.P238TPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-5.3-GFP-HiBit
Plasmid#171014PurposeFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-5.3-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Grin1 gRNA#3
Plasmid#169791PurposeCas9-mediated knockout of Grin1 in mammalian cellsDepositorInsertGrin1 (Grin1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Grin1 gRNA#2
Plasmid#169790PurposeCas9-mediated knockout of Grin1 in mammalian cellsDepositorInsertGrin1 (Grin1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#1
Plasmid#170834PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tbx21 gRNA#2
Plasmid#170838PurposeCas9-mediated knockout of Tbx21 in mammalian cellsDepositorInsertTbx21 (Tbx21 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Limk1 gRNA#3
Plasmid#163393PurposeCas9-mediated knockout of Limk1 in mammalian cellsDepositorInsertLimk1 (Limk1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tbx21 gRNA#3
Plasmid#170839PurposeCas9-mediated knockout of Tbx21 in mammalian cellsDepositorInsertTbx21 (Tbx21 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tbx21 gRNA#1
Plasmid#170837PurposeCas9-mediated knockout of Tbx21 in mammalian cellsDepositorInsertTbx21 (Tbx21 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Limk1 gRNA#2
Plasmid#163392PurposeCas9-mediated knockout of Limk1 in mammalian cellsDepositorInsertLimk1 (Limk1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#2
Plasmid#170835PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#3
Plasmid#170836PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Grin1 gRNA#1
Plasmid#169789PurposeCas9-mediated knockout of Grin1 in mammalian cellsDepositorInsertGrin1 (Grin1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Venus-Actb
Plasmid#169788PurposeAn actin FRET sensor. Must be expressed with ECFP-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-PRGRH-Nluc
Plasmid#158608PurposePlasmid expressing PRG(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-null (BPK1077)
Plasmid#171128PurposePlasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.DepositorInsertpCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterpCMV and T7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-p115RH-Nluc
Plasmid#158607PurposePlasmid expressing p115(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt
Plasmid#166993PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire
Plasmid#149690PurposeFor packaging and expression of Chronos-mT-Sapphire in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt
Plasmid#166986PurposeTests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt
Plasmid#166991PurposeTests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(4), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt
Plasmid#166990PurposeTests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(1), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt
Plasmid#166987PurposeTests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(4), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt
Plasmid#166995PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn
Plasmid#159503PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-3_PolyU(1)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(7)_Tornado-Corn
Plasmid#159493PurposeTests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(7)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(4)_Tornado-Corn
Plasmid#159490PurposeTests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(4)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only