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Showing: 4281 - 4320 of 7545 results

  2. pET21-pelB-LaG-9

    Plasmid
    #172742
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-9
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172742-plasmid-map-sequence-id-340182

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  11. pET21-pelB-LaG-8

    Plasmid
    #172761
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-8
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172761-plasmid-map-sequence-id-340116

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  12. pET21-pelB-LaG-5

    Plasmid
    #172760
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-5
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172760-plasmid-map-sequence-id-340022

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  14. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  17. GFP-SP110A

    Plasmid
    #65392
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Article
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Insert
    SP110A
    Use
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pME-H2B

    Plasmid
    #108883
    Purpose
    Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)
    Depositor
    Rob Parton
    Article
    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do
    Insert
    H2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
    Use
    Multisite gateway
    Tags
    Expression
    Mutation
    human amino acid sequence but different codon usa…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    108883-plasmid-map-sequence-id-210105

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  35. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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  39. pET21-pelB-LaG-2

    Plasmid
    #172739
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-2
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172739-plasmid-map-sequence-id-340192

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  40. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid
    #84583
    Purpose
    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding
    Depositor
    Geert van den Bogaart
    Article
    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do
    Insert
    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin-homology domain, residues 209-306, mut…
    Promoter
    CMV
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 4281 - 4320 of 7545 results