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Plasmid#89282PurposeEntry vector containing TaCas9-nos terminator between attL1 and attR5 sitesDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-rat-PTP1B
Plasmid#35987DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsHis and Xpress Epitope TagExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LBR-mRFP1-polyA
Plasmid#59749PurposeExpress LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.DepositorInsertLBR-mRFP1
UseTagsmRFP1ExpressionMammalianMutationaa 1-249 of LBRPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mTFP-NLS-blast
Plasmid#136257PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmTFP-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
hME-pOX
Plasmid#178181PurposeProduce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytesDepositorInserthuman MCU-EMRE fusion protein
UseTags1D4ExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB6HindIII-rat FcRn
Plasmid#121671PurposeExpresses rat neonatal Fc receptor in mammalian cellsDepositorInsertrat neonatal Fc receptor (Fcgrt Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ELYS-emerin-EGFP-polyA
Plasmid#59745PurposeExpress ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertELYS-emerin-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorArticleInsertyeGFP
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterpGRR-r11-r10Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterADH1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTS-HA-GFP11
Plasmid#177725PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA taggedDepositorInsertMTS-HA-GFP11
UseTagsGFP11 and HAExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS_miR-146aSponge_mCherry_GFP
Plasmid#149718PurposeLentiviral vector expressing a miR-146a decoy spongeDepositorInsert14X miR-146a binding sites
UseLentiviralTagsExpressionMammalianMutationG to T mutation in position 75 of the insertPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHD-mW-attPCC-FRT (pTL717)
Plasmid#115158PurposeVector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.DepositorInsertmW attP-CC FRT
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTrc99-Rat FABP2
Plasmid#13578DepositorInsertintestinal fatty acid binding protein (Fabp2 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJL6
Plasmid#184134PurposeFor labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.DepositorInsertCELE_VW02B12L.1 (vha-6 Nematode)
UseTagsRFPExpressionWormMutationPromoterPvha-6 (894bp)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI. PGK-puro
Plasmid#136266PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletIExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV Cyclin D2-long-mut
Plasmid#21657DepositorInsertCyclin D2 (CCND2 Human)
UseRetroviralTagsExpressionMammalianMutationmutated miR-15/16 and let-7 binding sitesPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSV-p65T305A,S276A
Plasmid#106456PurposeNF-kB p65, kinase sites 276,305 mutatedDepositorInsertNF-kB p65, kinase sites 276,305 mutated (RELA Human)
UseTagsExpressionMammalianMutationkinase sites inactivatedPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only