We narrowed to 8,887 results for: plat

1. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR-E

   Plasmid

   #78852

   Purpose

   Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

   Depositor

   Phillip Abbosh

   Article

   Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Cas9-P2A-Puro

   Expression

   Mammalian

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLJR962

   Plasmid

   #115162

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHJ12: CadC-VHH-Caffeine (No linker)

   Plasmid

   #108244

   Purpose

   Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
   

   Use

   Synthetic Biology

   Promoter

   pLacO1

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

   Plasmid

   #214875

   Purpose

   Empty lentiviral backbone for guide array cloning

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204341

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-i3/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMTL5

   Plasmid

   #127967

   Purpose

   DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TPP1-bio-His

    Plasmid

    #52090

    Purpose

    Expresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TPP1 (TPP1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    GPDp

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-CMV-Galpha-i3 ONE-GO

    Plasmid

    #189737

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i3 ONE-GO
    GNAI3 (Gnai3 Rat)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid

    #227246

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1α and pTRE3G

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Puro_siKD

    Plasmid

    #86695

    Purpose

    Tetracycline inducible expression of shRNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

    Plasmid

    #183150

    Purpose

    Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Inserts

    Lex-ER-B112
    Zif268 DBD - hPRLBD - MSN2AD
    

    Use

    For yeast genomic integration

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDM1383

    Plasmid

    #207423

    Purpose

    Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

    Depositor

    Rob Kay

    Article

    Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

    Inserts

    HSPC300
    Raf1 (RAF1 Human)
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Mutation

    aa 1–134 (RBD) only

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMEH31 Lamp2b HA

    Plasmid

    #168208

    Purpose

    Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-PKCdelta-EGFP

    Plasmid

    #178856

    Purpose

    Mammalian expression of PKCδ fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ (PRKCD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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