We narrowed to 8,892 results for: plat

1. pGMC00028 (aka pTS0057)

   Plasmid

   #200832

   Purpose

   AAVS1 guide RNA cloned into the lentiSAM puro backbone

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Insert

   AAVS1 guide RNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115219

   Purpose

   Expresses PspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PspCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. THBS1-bio-His

   Plasmid

   #53417

   Purpose

   Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   THBS1 (THBS1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPR-E

   Plasmid

   #78852

   Purpose

   Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

   Depositor

   Phillip Abbosh

   Article

   Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Cas9-P2A-Puro

   Expression

   Mammalian

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TERTpWT_Gluc_CCR5

   Plasmid

   #192270

   Purpose

   Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Promoter

   TERT promoter driving expression of gaussia

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLJR962

   Plasmid

   #115162

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

    Plasmid

    #214875

    Purpose

    Empty lentiviral backbone for guide array cloning

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204341

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-i3/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. TPP1-bio-His

    Plasmid

    #52090

    Purpose

    Expresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TPP1 (TPP1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-CMV-Galpha-i3 ONE-GO

    Plasmid

    #189737

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i3 ONE-GO
    GNAI3 (Gnai3 Rat)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid

    #227246

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1α and pTRE3G

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    GPDp

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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