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Plasmid#182354PurposeNuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11061
Plasmid#68255PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Gm
Plasmid#222335Purposeflp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3DepositorUseTagsExpressionMutationPromotercl, flp, and sacBAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 GFP V3
Plasmid#226961PurposeCBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG459 V3
Plasmid#226958PurposeCBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458 eSp(1.1) V3
Plasmid#226963PurposeCBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 GFP V3
Plasmid#226962PurposeCBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG458 V3
Plasmid#226959PurposeCBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 puro V3
Plasmid#226960PurposeCBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458 V3
Plasmid#226957PurposeCBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-miRFP670-TRF1
Plasmid#227378PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, WT (TERF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-9YS-miRFP670-TRF1
Plasmid#227381PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 9YS (TERF1 Human)
UseLentiviralTagsExpressionMutation9YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-27YS-miRFP670-TRF1
Plasmid#227383PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 27YS (TERF1 Human)
UseLentiviralTagsExpressionMutation27YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
iLID-miRFP670-TRF1
Plasmid#227385PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric iLID (TERF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-15YS-miRFP670-TRF1
Plasmid#227382PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 15YS (TERF1 Human)
UseLentiviralTagsExpressionMutation15YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only