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Plasmid#214430PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7DepositorInsertSMN2 EXON 7 (SMN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
HypoxCR
Plasmid#59946PurposeA lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.DepositorInsertsGFP-Pest
mCherry-Geminin
UseLentiviralTagsFLAG and PESTExpressionMammalianMutationPromoterCMV and min CMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pĪ»PR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM75
Plasmid#133649PurposeIntegration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-ANP1-mCherry (ANP1 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
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prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
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ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-ANP1-GFP (ANP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
UseTagsExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEn-C1.1
Plasmid#61479PurposeEncodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPCDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3 containing mRFP1
Plasmid#118083PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorInsertdopamine transporter (slc6a3 Zebrafish)
UseZebrafish transgenesisTagsEGFPExpressionMutationPromoterdatAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-Cas9-gate
Plasmid#113742PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.DepositorInsertCas9
UseCRISPR; Gateway destination vectorTagsExpressionMutationPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A-TelR
Plasmid#204979PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter speā¦PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS16 Pmyo-3 NPP-9 mChBioFLAG
Plasmid#45099DepositorUseTagsBLRP, FLAG, and mCherryExpressionWormMutationPromotermyo-3 and unc-119Available sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only