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Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NeuA.pJL1
Plasmid#199115PurposeIn vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)DepositorInsertN-acylneuraminate cytodiylyltransfease (NeuA)
UseTagsStrep-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.539
Plasmid#184958PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
UseTagsExpressionBacterialMutationfabH donor G954APromoterT7/lacAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
UseTagsExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
UseTagsExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC4 Grin1-smFP-V5 KI
Plasmid#183439PurposeFlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)DepositorInsertgRNA and smFP V5 donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xHA-Syn-smFP-flag
Plasmid#182563PurposeFor mouse Nav1.6 knockin with 3xHA at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xHA donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-HaloTag-V5-EF1-sfGFP
Plasmid#182565PurposeFor mouse Nav1.6 knockin with HaloTag-V5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xV5-EF1-smFP-flag
Plasmid#182562PurposeFor mouse Nav1.6 knockin with 3xV5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-pleCopyCatcher
Plasmid#174061PurposeCopyCatcher insertion donor for the pale locusDepositorInsertPale (Pale Synthetic, Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Hsa-Serum Albumin SP
Plasmid#153496PurposeHuman Serum Albumin Signal Peptide Donor PlasmidDepositorInsertSerum Albumin SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Killer Protein SP
Plasmid#153498PurposeS. cerevisiae Killer Signal Peptide Donor PlasmidDepositorInsertKiller Protein SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cfo-CPO SP
Plasmid#153500PurposeCaldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor PlasmidDepositorInsertChloroperoxidase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cgl-UPO SP
Plasmid#153501PurposeChaetomium globosum Unspecific Peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cci-UPO SP
Plasmid#153502PurposeCoprinopsis cinerea Unspecific Peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Ani- α Amylase SP
Plasmid#153503PurposeAspergillus niger α Amylase Signal Peptide Donor PlasmidDepositorInsertα Amylase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce- α Galactosidase SP
Plasmid#153504Purposeα Galactosidase Signal Peptide Donor PlasmidDepositorInsertα Galactosidase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Aae-UPO* SP
Plasmid#153506PurposeAgrocybe aegerita Unspecific peroxygenase (UPO*) Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase* SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Mth-UPO SP
Plasmid#153507PurposeUnspecific peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only