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Plasmid#177800PurposeBacterial expression of green fluorescent potassium indicator KRaION2DepositorInsertKRaION2
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidTagsExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…PromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-R-GECO1
Plasmid#32465DepositorArticleInsertR-GECO1.0
UseLab constructedTags6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationSubstitutions relative to the mApple-derived anal…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD WT TET1
Plasmid#124082PurposeExpression of wild-type catalytic domain only form of TET1DepositorInsertTET1 catalytic domain (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD MUT TET1
Plasmid#124083PurposeExpression of mutated catalytic domain only form of TET1DepositorInsertTET1 - catalytic domain mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-IRES-Puro
Plasmid#58971Purposenoninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorTypeEmpty backboneUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-JEDI-1P-WPRE
Plasmid#202615PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3
Plasmid#48743PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +1060)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-CEN_ARS_HIS
Plasmid#202129PurposeLevel 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.DepositorInsertCEN_ARS_HIS
UseSynthetic BiologyTagsExpressionMutationARS sequence mutated to remove sapI site for dome…PromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTiGc
Plasmid#78314PurposeMycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).DepositorInsertsTurboFP635
GFP
UseSynthetic Biology; Mycobacterial replicating vect…TagsExpressionBacterialMutationPromoterhsp60 and hsp60(ribo)Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-RexYFP
Plasmid#141070PurposeBacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratioDepositorInsertRexYFP
UseTags6xHisExpressionBacterialMutationchanged Leucine 169 to Proline, changed Tyrosine …PromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP3bright
Plasmid#74423PurposeExpress fluorescent calcium indicator GCaMP3bright in mammalian cellsDepositorInsertGCaMP3bright
UseTagsExpressionMammalianMutationD392APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
ECFP HO
Plasmid#207413PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP CD
Plasmid#207414PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano50/pcDNA3
Plasmid#51964Purposemammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nMDepositorInsertYellow Cameleon-Nano50
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNA-leu-HYB
Plasmid#64332Purposeleu2 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of leu2 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP3fast
Plasmid#74422PurposeExpress fluorescent calcium indicator GCaMP3fast in mammalian cellsDepositorInsertGCaMP3fast
UseTagsExpressionMammalianMutationW4Y D356APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only