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Plasmid#80048PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01(L33R)
UseTagsMycExpressionMammalianMutationL33RPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPJ169(JPUB_006769)
Plasmid#83835PurposeCarries NEW operon 5 finalDepositorInsertsKuste3340
Kuste3350
UseTagsExpressionMutationPromoterop5_3350_for and op5_BsaI_3340_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ170(JPUB_006771)
Plasmid#83833PurposeCarries NEW operon 6 finalDepositorInsertsKuste3346
Kuste3348
Kuste3349
UseTagsExpressionMutationPromoterop6_3348_for, op6_3349_for, and op6_BsmBI_3346_fo…Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ168(JPUB_006767)
Plasmid#83832PurposeCarries NEW operon 4 finalDepositorInsertsKuste2802
Kuste2805
UseTagsExpressionMutationPromoterop4_2805_for and op4_BsaI_2802_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_GB
Plasmid#78677PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_FB
Plasmid#78676PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_EB
Plasmid#78675PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_AB
Plasmid#78674PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWK
Plasmid#62713PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInsertamiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pARA (pKD-G1)
Plasmid#62680PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pARB (pKD-G2)
Plasmid#62681PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pARD (pKD-G4)
Plasmid#62683PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATJ (pKD-G8)
Plasmid#62687PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-SupH
Plasmid#59710PurposepSALECT with SupH insertDepositorInsertSupH
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-AhyI
Plasmid#59708PurposepSALECT with AhyI insertDepositorInsertAhyI
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST myc Rab35 CA
Plasmid#58382Purposeexpression of drosophila myc-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagsmycExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab35 CA
Plasmid#58381Purposeexpression of drosophila YFP-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2 short
Plasmid#51596Purposeexpresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)DepositorInsertRem2 (Rem2 Rat)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only