Addgene

We narrowed to 8,408 results for: CAG

Showing: 2121 - 2140 of 8408 results

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pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

Plasmid

#218881

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Insert

iGABASnFR2n

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC3-pA

Plasmid

#220755

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC3

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC7-pA

Plasmid

#220759

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC7

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

Plasmid

#218869

Purpose

Mammalian expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Insert

iGABASnFR2n

Use

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

Plasmid

#178518

Purpose

For making Rabies virus SAD B19 strain L cell line with hyPBase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

B19L-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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p194-RV-CAG-dio-mScarlet-T2A-Brn2

Plasmid

#204741

Purpose

Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Brn2

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p186-RV-CAG-dio-mScarlet-T2A-mVenus

Plasmid

#204740

Purpose

Conditional expression of target gene mVenus with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mVenus

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-9-SV40 polyA

Plasmid

#190873

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode9

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

Plasmid

#198069

Purpose

Mammalian expression of iRFP713(C15S/V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(C15S/V256C)

Use

Tags

P2A-mCherry

Expression

Mammalian

Mutation

C15S/V256C mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D908A

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-7-SV40 polyA

Plasmid

#190871

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode7

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-8-SV40 polyA

Plasmid

#190872

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode8

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-6-SV40 polyA

Plasmid

#190870

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode6

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE

Plasmid

#186049

Purpose

AAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

LifeAct-jGCaMP8s

Use

AAV

Tags

LifeAct-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (801-839)

Plasmid

#188089

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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