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Showing: 2121 - 2140 of 6831 results

  1. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  2. pCSIIhyg-rtTA

    Plasmid
    #139480
    Purpose
    Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Insert
    rtTA, Tet-ON reverse transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139480-plasmid-map-sequence-id-276014

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  3. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  4. P[acman]-Alpha2

    Plasmid
    #165859
    Purpose
    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165859-plasmid-map-sequence-id-336455

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  5. pGL4.R_TRE_minPro

    Plasmid
    #211517
    Purpose
    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
    Depositor
    Justin English
    Article
    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    SV40 and minPro
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211517-plasmid-map-sequence-id-419251

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  16. pGL4.R_TRE_minTK

    Plasmid
    #211516
    Purpose
    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
    Depositor
    Justin English
    Article
    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    SV40 and minTK
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211516-plasmid-map-sequence-id-419246

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Showing: 2121 - 2140 of 6831 results