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Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
UseTagsExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_NSD2
Plasmid#221376PurposeExpresses NSD2 in bacterial cellsDepositorInsertHistone-lysine N-methyltransferase NSD2 (NSD2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX_K1033R
Plasmid#221378PurposeExpresses ATRX_K1033R in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionBacterialMutationChanged lysine 1033 to argininePromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM
Plasmid#221379PurposeExpresses FANCM in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM_K819R
Plasmid#221380PurposeExpresses FANCM_K819R in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionBacterialMutationChanged lysine 819 to argininePromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEYFP_C1_ATRX
Plasmid#221383PurposeExpresses ATRX in mammalian cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionMammalianMutationK536N (Please see depositor comments)PromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSK519-cspB
Plasmid#207162PurposeExpresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vectorDepositorInsertcspB
UseTagsHAExpressionBacterialMutationPromoternptIIAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-myr-rsEGFP2- LDLR
Plasmid#213399Purposedouble floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2DepositorInsertrsEGFP2
UseAAVTagsC-terminal (Ct) cytoplasmic domains of low densit…ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
IAA17-4flag-HIS5
Plasmid#207015PurposeFor ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-HIS5
Plasmid#207014PurposePlasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-MET15
Plasmid#207013PurposePlasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-URA3
Plasmid#207012PurposePlasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10D(E215Q)-HA
Plasmid#209248PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseTagsHAExpressionMammalianMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10D(E215Q)-HA
Plasmid#209260PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseRetroviralTagsHAExpressionMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10B(E210Q)-HA
Plasmid#209247PurposeMammalian expression of ATP10B mutant E210QDepositorInsertATP10B (ATP10B Human)
UseTagsHAExpressionMammalianMutationE210QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10B-HA
Plasmid#209233PurposeMammalian expression of ATP10BDepositorInsertATP10B (ATP10B Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10B(E210Q)-HA
Plasmid#209259PurposeMammalian expression of ATP10B mutant E210QDepositorInsertATP10B (ATP10B Human)
UseRetroviralTagsHAExpressionMutationE210QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10A-HA
Plasmid#209232PurposeMammalian expression of ATP10ADepositorInsertATP10A (ATP10A Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2
Plasmid#203605PurposeReporter plasmid carrying PHLPP2 3'UTRDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-27bT
Plasmid#203600PurposeReporter plasmid carrying miR-27b target sequenceDepositorInsertcomplementary sequence to miR27b-3p (MIR27B Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR
Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
Plasmid#201534PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB3
UseSynthetic BiologyTagsExpressionMutationrhaS W190GPromoterPrhaBAD (rhamnose)Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15a_AcCas12n
Plasmid#203806Purposep15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.DepositorInsertAcCas12n
UseCRISPRTagsExpressionMutationPromoterrpslAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mRuby3-2A-dCre
Plasmid#203845PurposeFlp-dependent enzymatically inactive Cre and mRuby3 expressionDepositorInsertdCre
UseAAVTagsmRuby3-2AExpressionMutationEnzymatically inactive CrePromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PV1_VP1
Plasmid#202071PurposeBacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertPV1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVA4_VP1
Plasmid#202068PurposeBacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVA4 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyTagsExpressionMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyTagsExpressionMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertEXPANSIN A10 (EXPA10 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterEXPA10Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-domain
Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-HiBIT-WDR5
Plasmid#200883PurposeBacterial expression of N-terminal HiBIT-tagged human WDR5 proteinDepositorInsertHiBIT-WDR5 (WDR5 Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- BAD-YFP
Plasmid#186567PurposepMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC
Plasmid#148386PurposeInsect Expression of DmMe31B-QHTRKKtoAAAAAADepositorInsertDmMe31B-QHTRKKtoAAAAAA (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-R307AK313AK314A_AC
Plasmid#148387PurposeInsect Expression of DmMe31B-R307AK313AK314ADepositorInsertDmMe31B-R307AK313AK314A (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-trc- YFP
Plasmid#186569Purposep15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-T7- YFP
Plasmid#186568Purposep15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjT-tdTomato-PGK-Neo
Plasmid#186173PurposeKnock-in gene targeting in marmoset cells at TBXT locusDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-tdTomato-PGK-Neo
Plasmid#186172PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only