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Plasmid#46261PurposeBacterial targetingDepositorTypeEmpty backboneUseBacterial targetingTagsExpressionMutationPromoterAvailable sinceAug. 14, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits
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pDule-para-aminoPhe
Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3485
Plasmid#144961PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF2 (ATF2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
huCaV2.2 (pSAD442-1)
Plasmid#62574PurposeHuman CaV2.2 expression in mammalian cellsDepositorInsertCACNA1B (CACNA1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro mouse IRS-1 myc
Plasmid#11374DepositorInsertIRS-1 (Irs1 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
RP4-7
Plasmid#79811PurposeModified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmidDepositorInsertChloramphenicol acetyltransferase
UseHelper plasmid for mobilizationTagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1
Plasmid#218796PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmAcK3RS(IPYE)
Plasmid#104071PurposeExpression plasmid encoding MmAcK3RS(IPYE) and pylTDepositorInsertPlpp MmAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADV-Sec61b-TurboID
Plasmid#179143PurposeRecombinant adenoviral plasmid for Sec61b-TurboID expressionDepositorInsertSec61b-V5-TurboID
UseAdenoviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMN-334
Plasmid#195468PurposeLentiviral Integratable Inducible synZiFTR ZF10 + ReporterDepositorInsertinverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IDH1
Plasmid#116751PurposeLentiviral expression of IDH1DepositorInsertIDH1 (IDH1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD WT
Plasmid#169713PurposeBacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-FLIM-AKAR
Plasmid#60445PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKAR
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.V600E
Plasmid#82186PurposeGateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationV600EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY016 (pcDNA3.1-hLbCpf1)
Plasmid#69988PurposeExpresses humanized LbCpf1DepositorInserthLbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
UseTagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTet-IRES-EGFP
Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0
Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR1
Plasmid#116740PurposeLentiviral expression of FGFR1DepositorInsertFGFR1 (FGFR1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II
Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IDH1-R132C
Plasmid#116410PurposeLentiviral expression of IDH1 R132CDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTagsExpressionMutationR132CPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Neo
Plasmid#60525PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOS017: ratiometric pHluorin (cytosolic)
Plasmid#163052Purposeratiometric pHluorin (cytosolic)DepositorInsertratiometric pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-HA-gate-pGK-HYG
Plasmid#107396PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-myc-CAR-2A-Thy1.1
Plasmid#127890PurposeExpresses CART receptor along with tags.DepositorInsertsmyc,
CAR
2A
Thy1.1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI NL4-3 Nef-HA-WT
Plasmid#24162DepositorInsertNef (nef HIV)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MCS-GFP-SV-puro
Plasmid#73582PurposeThird generation lentiviral vector expressing GFP and puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsGFP tagExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VARP
Plasmid#42312DepositorInsertVARP (ANKRD27 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only