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pCLBW cox8 EGFP mCherry

Plasmid

#78520

Purpose

Fluorescent mitophagy reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

none

Use

Retroviral

Tags

cox8 EGFP mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEcgRNA

Plasmid

#166581

Purpose

CRISPR-Cas9-assisted genome editing in Escherichia coli

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8m

Plasmid

#162372

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Use

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLux and pTet

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Arf1-GFP

Plasmid

#39554

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylation factor 1 (ARF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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