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pLib6.4-Aaeg_774

Plasmid

#176659

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Agam_695

Plasmid

#176668

Purpose

Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15415-BEAR-GFP-target-mCherry

Plasmid

#162995

Purpose

plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUFM1

Plasmid

#86133

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFM1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFM1 (UFM1 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-NLS-tdMCP-mCherry

Plasmid

#99909

Purpose

For the expression of NLS-tdMCP-mCherry

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-tdMCP-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/EGFP/pdCas9-C1

Plasmid

#162759

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT448

Plasmid

#139388

Purpose

Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Bile Acid inducible Promoter

Available since

Jan. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMT450

Plasmid

#139390

Purpose

Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Bile Acid inducible Promoter

Available since

Jan. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT492

Plasmid

#139397

Purpose

Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Bile Acid inducible Promoter

Available since

Jan. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pV1539

Plasmid

#111434

Purpose

Genedrive entry vector for Candida albicans

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1464

Plasmid

#111440

Purpose

N. castellii Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCIP-mB2M

Plasmid

#154091

Purpose

Self-Cutting and Integrating CRISPR backbone targeting murine B2M

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting murine B2M locus

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-NLS-tdPCP-EGFP

Plasmid

#99910

Purpose

For the expression of NLS-tPMCP-EGFP

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-tdPCP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE4G

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_744

Plasmid

#176663

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgUFSP2

Plasmid

#86134

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFSP2 (UFSP2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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SGL40C-H1.EFS.RFP657

Plasmid

#69148

Purpose

Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Use

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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