Addgene

We narrowed to 27,415 results for: eng

Showing: 2101 - 2120 of 27415 results

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negMaLionB

Plasmid

#113911

Purpose

negative control for Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3-ST

Plasmid

#129262

Purpose

Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3-ST

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTX179

Plasmid

#89261

Purpose

Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCBEclos-cbei2385-g2-opt

Plasmid

#118215

Purpose

CRISPR-mediated base editing in Clostridium

Depositor

Sheng Yang

Article

Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

Insert

Apobec1, Casp nickase, UG1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptm

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK150

Plasmid

#183126

Purpose

BTK Type 2 promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Use

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-HsTRBP

Plasmid

#41096

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

TRBP (TARBP2 Human)

Use

Tags

6xHis-HRV3Csite

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pT2-Tbait-UAS-Positron-ST

Plasmid

#129266

Purpose

UAS-driven expression of Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Tags

Expression

Mutation

D92N, E199V

Promoter

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTX172

Plasmid

#89259

Purpose

Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_S-Luc_CLIP

Plasmid

#113923

Purpose

Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_CLIP

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTX176

Plasmid

#89260

Purpose

Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTX168

Plasmid

#89257

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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POT5-TEF2p-ycrtYB-ADH1t

Plasmid

#65359

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtYB-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TEF2p-ycrtE-ADH1t

Plasmid

#65353

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtE-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-TDH3p-ycrtI-ADH1t

Plasmid

#65355

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtI-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJAID8Z104

Plasmid

#165056

Purpose

Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDTN3997

Plasmid

#113337

Purpose

Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

multi-copy 2-pyrone synthase

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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POT5-CYC1p-ycrtYB-ADH1t

Plasmid

#65360

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtYB-ADH1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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