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pFETCh_SOX5
Plasmid#86292PurposeDonor vector for 3' FLAG tag of human SOX5DepositorInsertSOX5 homology arms (SOX5 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pFETCh_ZNF644
Plasmid#86264PurposeDonor vector for 3' FLAG tag of human ZNF644DepositorInsertZNF644 homology arms (ZNF644 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_NKX2-1_HD_160-223AA
Plasmid#221477PurposeGateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
UseTagsNoneExpressionMammalianMutationPromoterSV40Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only
