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Plasmid#167279PurposeCodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only
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pHDR-USR-eGFP
Plasmid#208830PurposeHDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMV, U6Available sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-6
Plasmid#72346PurposeMammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-AcrIIA4
Plasmid#113037PurposeExpression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PUM1_PUM_pGEX
Plasmid#135129PurposeRecombinant expression of PUM1 with dual-affinity tagDepositorInsertPUM1 (PUM1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
UseTagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABE8eHD-BlastR
Plasmid#176478PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABE8eHD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP Nup62Fv2GFP
Plasmid#139684PurposeNup62 dimerization construct fused to 2 copies of GFP under an SFFV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2
Plasmid#72216Purposebacterial expression of GST-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationF146Y and E198K (please see depositor's comm…PromotertacAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SRSF8_RRM_pGEX
Plasmid#135119PurposeRecombinant expression of SRSF8 with dual-affinity tagDepositorInsertSRSF8 (SRSF8 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pNB23
Plasmid#103058Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGpDepositorInsertGFP-OVA
UseTagsExpressionBacterialMutationPromoterhydrogen peroxide-inducible promoter katGpAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW6.01
Plasmid#62882PurposeTransgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTRDepositorInsertGFP::MEG-3
UseTagsGFPExpressionWormMutationPromotermeg-3Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE347
Plasmid#153228PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-1
Plasmid#72343PurposeMammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE668
Plasmid#153233PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only