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pCas27

Plasmid

#82384

Purpose

sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23117

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas15

Plasmid

#82383

Purpose

sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas14

Plasmid

#82382

Purpose

sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas13

Plasmid

#82381

Purpose

sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pOGGUSPlus

Plasmid

#64400

Purpose

Oat globulin Glo1 promoter driving expression of GUSPlus

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat globulin Glo1 promoter

Use

Tags

GUSplus (modified Staphylococcus sp. b-glucuronid…

Expression

Plant

Mutation

Promoter

Oat globulin Glo1

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
pOGGFPR

Plasmid

#64398

Purpose

Oat globulin Glo1 promoter driving expression of GFP

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat globulin Glo1 promoter

Use

Tags

GFP

Expression

Plant

Mutation

Promoter

Oat globulin Glo1

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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UAS-Myc-dASUN

Plasmid

#47036

Purpose

Drosophila expression of tagged dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

Myc

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
TV3-mCHY-dASUN

Plasmid

#47034

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

mCHY

Expression

Insect

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
TV3-eGFP-dASUN

Plasmid

#47033

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pLG-Nde-Acc

Plasmid

#33150

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

AccI site in intron was cut/filled/ligated to cha…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE E171Q HDEL

Plasmid

#32124

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, E171Q

Promoter

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE K108A GFP

Plasmid

#32126

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE K108A HDEL

Plasmid

#32122

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pRT498-3xOtUBD

Plasmid

#190088

Purpose

Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

3xOtUBD

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-eGFP-dspB(E184Q)-6xHis

Plasmid

#175783

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Use

Tags

Hexahistidine tag and eGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-BmAgo3

Plasmid

#50559

Purpose

Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

BmAgo3

Use

Tags

6xHIS and Flag

Expression

Insect

Mutation

Promoter

OpIE2

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD

Plasmid

#190089

Purpose

Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge