Addgene

We narrowed to 3,927 results for: KRAS

Showing: 2101 - 2120 of 3927 results

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pcDNA5FRT/TO-DVC1-Strep-HA

Plasmid

#113481

Purpose

Expression of human DVC1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

DVC1 (SPRTN Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V2

Plasmid

#19968

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-C terminal Venus (DRD2 Aequorea Victoria, Human)

Use

Tags

C terminal Venus and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-HOIP-Strep-HA

Plasmid

#113494

Purpose

Expression of human HOIP with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

HOIP (RNF31 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

QUASM1-E1b

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLIX402 caPHF2

Plasmid

#91701

Purpose

inducible lentiviral expression of constitutively active PHF2

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

caPHF2; S757D, S899D, S954D, S1056D

Promoter

TRE promoter, Tet ON

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-cGASΔN-HA (murine)

Plasmid

#130915

Purpose

Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN murine

Use

Tags

HA

Expression

Mammalian

Mutation

murine cGAS catalytic domain (aa148-507)

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-7

Plasmid

#55399

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Alpha-5-Integrin-12

Plasmid

#55401

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Alpha-5 Integrin (ITGA5 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Strep/HA-UBE2Q2

Plasmid

#124668

Purpose

Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2Q2 (UBE2Q2 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Clathrin-15

Plasmid

#55408

Purpose

Localization: Clathrin Vesicles, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Clathrin (CLTB Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-UBXD1-Strep-HA

Plasmid

#113476

Purpose

Expression of human UBXD1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD1 (UBXN6 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

C174T silent mutation, wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-N-HA (murine)

Plasmid

#130916

Purpose

Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

murine cGAS N terminus

Use

Tags

HA

Expression

Mammalian

Mutation

murine cGAS N terminus (aa 1-147)

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-CD36-C-10

Plasmid

#55405

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

CD36 (CD36 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
eGFP-pk-H302A

Plasmid

#115618

Purpose

Expresses human Parkin H302A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H302A

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15416-BEAR-mScarlet-target-BFP

Plasmid

#162996

Purpose

plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-mScarlet plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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625_HOXD13 in pmiRGlo

Plasmid

#78129

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

HOXD13 (HOXD13 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Vimentin-7

Plasmid

#55452

Purpose

Localization: Intermediate Filaments, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vimentin (VIM Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only