Addgene

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pEBB-GFP-hBEN-deltaSS

Plasmid

#22159

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 897-908

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEBG-hBEN-deltaNLS3

Plasmid

#22161

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Deletion of amino acids 883-889

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEBG-hBEN-deltaSS

Plasmid

#22162

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Deletion of amino acids 897-908

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

Plasmid

#79379

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

Plasmid

#79382

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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GFP-Mff

Plasmid

#49153

Purpose

mammalian expression of Mff fused to GFP

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

Mff (MFF Human)

Use

Tags

acGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265E2

Plasmid

#164719

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-WT-STAT1

Plasmid

#71454

Purpose

Lentiviral expression of WT-STAT1

Depositor

George Stark

Article

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL14

Plasmid

#53740

Purpose

For expression of METTL14 (methyltransferase-like 14) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 14 (METTL14 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pmStrawberry-Atg4BC74A

Plasmid

#21076

Depositor

Tamotsu Yoshimori

Article

Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

Insert

Atg4B(C74A) (Atg4b Mouse)

Use

Tags

mStrawberry

Expression

Mammalian

Mutation

220-222 TGC(Cys) is changed to GCA (Ala)

Promoter

Available since

June 8, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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B-MFF

Plasmid

#141158

Purpose

Dimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Use

Tags

GB

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEIGW Grx1-roGFP2

Plasmid

#64990

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-Caveolin-C-10

Plasmid

#55008

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Stat1 alpha pRC/CMV

Plasmid

#8690

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 alpha (STAT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB_OT1.0

Plasmid

#185386

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pQTEV-LRPAP1

Plasmid

#31327

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

low density lipoprotein receptor-related protein associated (LRPAP1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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