-
Plasmid#14675DepositorInsertMKK7g1(K165A) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationLys-165AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS1-mutant Fzd3 3'UTR
Plasmid#25023DepositorInsertmutant Fzd3 3'UTR
UseLuciferaseTagsExpressionMammalianMutationmiR-31 binding site in 3’ UTR mutagenized from TC…PromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGS1011
Plasmid#63783PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection markerDepositorInsertStrep-tag II followed by TRP1 selection marker
UseE. coli cloning vectorTagsStrep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPVpr-hp
Plasmid#48119PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterDepositorInsertsignal peptide of Vpr
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1CH2AB
Plasmid#13475DepositorInsertMKP-1CH2AB (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationContains aa 1-136PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk3a2(2nd methione)
Plasmid#15747DepositorInsertJnk3a2(2nd methione) (MAPK10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab18 WT
Plasmid#37717DepositorInsertRab18 (Rab18 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFusX1-31
Plasmid#63249PurposeEncodes RVD positions 1-3 in TALEN assemblyDepositorInsertHD NG NN
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K651R
Plasmid#84528PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK651RPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSM15
Plasmid#101640PurposeEncodes the fluorescent protein EG-4 for constitutive expression in E. coli.DepositorInsertEG-4
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX3 (pcDNA3.1+)
Plasmid#75205PurposeMammalian expression of SLC24A3DepositorInsertSLC24A3 (SLC24A3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab8 WT
Plasmid#37705DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 WT
Plasmid#37696DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)
Plasmid#60245PurposeExpress the RBD of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains aa7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA4b delta6
Plasmid#47590DepositorInsertPMCA4bdelta6 (ATP2B4 Human)
UseTagsEGFPExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST39-His/Vps27(345-440) - Hse(275-375)
Plasmid#17638DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab18 DN
Plasmid#53474DepositorInsertRab18 (Rab18 Fly)
UseTagsYFPExpressionInsectMutationS19NPromoterGAL4Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab18 CA
Plasmid#37719DepositorInsertRab18 (Rab18 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationA64L (constitutive active)PromoterGAL4Available sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-31
Plasmid#63440PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertHD NG NN
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1DCH2B
Plasmid#13471DepositorInsertMKP-1DCH2B (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion of MKP-1 CH2B; Delta 47-137PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
5mCUC1 pBluescript
Plasmid#12065DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UseTagsExpressionBacterialMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
Syn3-27-146+TM
Plasmid#14679DepositorInsertSyntaxin3 (STX3 Human)
UseTags2xMyc-HisExpressionMammalianMutationInsert is cloned into a modified vector; aa 27-14…PromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab1 WT
Plasmid#37681DepositorInsertRab1 (Rab1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab3 WT
Plasmid#44820DepositorInsertRab3 (Rab3 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab14 WT
Plasmid#37714DepositorInsertRab14 (Rab14 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1CH2A
Plasmid#13473DepositorInsertMKP-1CH2A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationContains aa 1-46PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1CH2B
Plasmid#13474DepositorInsertMKP-1CH2B (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationContains aa 47-136PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1L16A
Plasmid#13476DepositorInsertMKP-1L16A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationChanged Leucine 16 to AlaninePromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1L17A
Plasmid#13477DepositorInsertMKP-1L17A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationChanged Leucine 17 to AlaninePromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
pBa.KIF13B
Plasmid#224413PurposeMammalian expression of Kif 13bDepositorInsertKif 13b (Kif13b Mouse)
UseTagsExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.KIF13A
Plasmid#224408PurposeMammalian expression of Kif 13aDepositorInsertKif 13a (Kif13a Mouse)
UseTagsExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.MARK2-Halo
Plasmid#224414PurposeMammalian expression of MARK2DepositorInsertMARK2 (MARK2 Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Syn1-mCherry
Plasmid#222967PurposeMake lentivirus to express mCherry in syn1 expressing cellsDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromotersynapsin IAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.Mark2-3myc
Plasmid#224416PurposeMammalian expression of MARK2DepositorInsertMARK2 (MARK2 Human)
UseTags3mycExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspB
Plasmid#215400PurposeCm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)DepositorInsertcspB
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA2
Plasmid#215399PurposeCm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)DepositorInsertcspA2
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PI4KB
Plasmid#221759PurposeStable expression of GFP-PI4KB in mammalian cellsDepositorInsertPI4KB (PI4KB Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PLCd1(PHD)
Plasmid#221753PurposeStable expression of GFP-PLCd1(PHD) in mammalian cellsDepositorInsertPLCd1 (PLCD1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–175PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-2xFYVE
Plasmid#221750PurposeStable expression of GFP-2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain
UseRetroviralTagsGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-WIPI2b-mRuby3
Plasmid#221749PurposeStable expression of WIPI2b-mRuby3 in mammalian cellsDepositorInsertWIPI2b (WIPI2 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-FAPP(PHD)
Plasmid#221748PurposeStable expression of GFP-FAPP(PHD) in mammalian cellsDepositorInsertFAPP (PLEKHA3 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–101PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-OSBP(PHD)
Plasmid#221745PurposeStable expression of GFP-OSBP(PHD) in mammalian cellsDepositorInsertOSBP (OSBP Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 87–185PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-STX17TM
Plasmid#221744PurposeStable expression of mRuby3-STX17TM in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationdeleted amino acids 1-228PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(Y557C)
Plasmid#209695PurposeExpresses MAC3-tagged CCDC22(Y557C) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
UseTagsMAC3-tagExpressionMammalianMutationY557C, changed Tyrosine 557 to CysteinePromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(T17A)
Plasmid#209694PurposeExpresses MAC3-tagged CCDC22(T17A) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
UseTagsMAC3-tagExpressionMammalianMutationT17A, changed Threonine 17 to AlaninePromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only