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Showing: 4201 - 4240 of 7545 results

  4. pPEPX-P3-sgRNAluc

    Plasmid
    #85590
    Purpose
    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    sgRNA targeting firefly luciferase encoding gene
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85590-plasmid-map-sequence-id-169513

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  7. p415 TEF Su9‐roGFP2‐Tsa2ΔCR

    Plasmid
    #83240
    Purpose
    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.
    Depositor
    Tobias Dick
    Article
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    Su9-roGFP2-Tsa2dCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCr with Su9 targetting se…
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83240-plasmid-map-sequence-id-164530

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  12. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  13. TPST2-EGFP

    Plasmid
    #66618
    Purpose
    Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)
    Depositor
    David Stephens
    Article
    Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do
    Insert
    TPST2 (TPST2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Insert contains T370A relative to NM_001008566.1
    Promoter
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pPBC-LG6s

    Plasmid
    #62809
    Purpose
    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62809-plasmid-map-sequence-id-123119

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Showing: 4201 - 4240 of 7545 results