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We narrowed to 47,817 results for: ANG

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

Plasmid

#170826

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

Plasmid

#170827

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

Plasmid

#170828

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

Plasmid

#170822

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-2

Plasmid

#210136

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-1

Plasmid

#210135

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22y/PfPRMT5-PTP

Plasmid

#203160

Purpose

for tagging PTP to the 5' end of PfPRMT5 gene by single crossover

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment, PTP tag and pDT 3' UTR

Use

Tags

3' UTR of P. berghei dihydrofolate reductase…

Expression

Mammalian

Mutation

Promoter

No

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-miniSdd3-BE4max-BlastR

Plasmid

#204849

Purpose

For cytosine base editing using miniSdd3 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGW02 AAV-TBG-Cre-sgP53-sgRNA

Plasmid

#192162

Purpose

AAV-TBG-Cre-sgP53-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-HA

Plasmid

#180145

Purpose

Coding sequence of human FAM3B pCS2 with C terminal HA tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-FLAG

Plasmid

#180146

Purpose

Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168244

Purpose

"Rescue Rac-CA expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-CA

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-DGCR8-2xdsRBD(+6)

Plasmid

#171542

Purpose

Encodes a DGCR8 fragment extending from E502 to the C-terminus

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DGCR8 (DGCR8 Human)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 RUNX1 g1

Plasmid

#155185

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d RUNX1 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-PIP1Δ

Plasmid

#160993

Purpose

Express mouse p150 protein with PIP1 deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

PIP1 (PCNA-interacting protein) motif deletion

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-Snrnp40

Plasmid

#134249

Purpose

Lentivector encoding Flag-tagged Snrnp40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-Sox2TALE-GCN4

Plasmid

#120548

Purpose

Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-NGN2TALE-GCN4

Plasmid

#120547

Purpose

Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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SHP2(WT)@pLKO-Trex-SBP

Plasmid

#85457

Purpose

Gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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SHP2(C459S)@pLKO-Trex-HA

Plasmid

#85459

Purpose

Expresses SHP2 mutant in mammalian cells

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

C459S

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
SHP2(T253M/Q257L)@pLKO-Trex-SBP

Plasmid

#85458

Purpose

SHP2 mutant gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

T253M/Q257L

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgFSP1

Plasmid

#186026

Purpose

knock out FSP1 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Ferroptosis Suppressor Protein 1 (AIFM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC9-C169S

Plasmid

#231673

Purpose

Expresses human ZDHHC9-C169S protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC9 (ZDHHC9 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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H3S10ph biosensor

Plasmid

#120809

Purpose

FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP

Use

Tags

Expression

Mammalian

Mutation

Threonine 3, Threonine 6, and Threonine 11 are mu…

Promoter

CMV

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only