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Plasmid#129668PurposeA repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
anti-FX(a) Emi HC
Plasmid#113665PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FX/FXa heavy chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
anti-FIX(a) Emi HC
Plasmid#113664PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FIX/FIXa heavy chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-HNF4A-FOXA2-FOXA3
Plasmid#149724Purposeexpressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)DepositorInsertHNF4A-FOXA2-FOXA3
UseLentiviralTagsP2A, T2AExpressionMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-IRF4-Puro
Plasmid#123323PurposeLentiviral vector for expression of IRF4 under a Doxycycline inducible promoterDepositorInsertIRF4 (IRF4 Human)
UseLentiviralTagsExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterDoxycycline inducible minimal CMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP hBub1 K821R
Plasmid#59813PurposeAllows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.DepositorInsertBub1 (BUB1 Human)
UseTagsEmGFPExpressionMammalianMutationK821RPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Climp63(1–192)
Plasmid#186955PurposeExpresses mCherry-Climp63 with partial luminal domainDepositorInsertCkap4 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationOnly remain partial luminal domainPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP hBub1 WT
Plasmid#59812PurposeAllows the integration of GFP hBub1 in the genome and Tet-inducible expression.DepositorInsertBub1 (BUB1 Human)
UseTagsEmGFPExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-GBP1-C589A
Plasmid#138090PurposeLentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)DepositorInserthuman GBP1 (GBP1 Human)
UseLentiviralTagsExpressionMammalianMutationcysteine 589 to alanine (C589A)Promoterminimal CMV promoter with heptamerized tet‐operat…Available sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX301-KANK1
Plasmid#121985Purposeoverexpress of KANK1DepositorInsertKANK1 (KANK1 Human)
UseLentiviralTagsExpressionMutationPromoterCMV promoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-WT-c-Src-FLAG
Plasmid#44652DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
UseTagsFLAG tag and mitochondria-targeting sequence (res…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-CA-c-Src-FLAG
Plasmid#44654DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
UseTagsFLAG tag and mitochondria-targeting sequence (MTS…ExpressionMammalianMutationchanged Tyr 530 to PhePromoterCMV promoterAvailable sinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-KD-c-Src-FLAG
Plasmid#44653DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
UseTagsFLAG tag and mitochondria-targeting sequence (MTS…ExpressionMammalianMutationchanged lysine 298 to methioninePromoterCMV promoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo/pTER shEGFP#2 (w22-2)
Plasmid#17480Purpose3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, NeoDepositorInsertEnhanced green fluorescent protein shRNA #2
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPKm-196
Plasmid#90511PurposepcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoterDepositorInsertPIF6-DBD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1
Plasmid#166711Purposeexpresses AMOT with inactivating mutation in first PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 L129A P130A
UseTags2xHAExpressionMammalianMutationchanged leucine 129 to alanine, proline 130 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/FLAG-hPMR1-Tev-Bio
Plasmid#80125PurposeExpresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
UseTagsFLAG and biotinylationExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Climp63(1–336)
Plasmid#186957PurposeExpresses mCherry-Climp63 with partial luminal domainDepositorInsertCkap4 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationOnly remain partial luminal domainPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only