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Plasmid#196828PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#174066PurposeEncodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal1-LacO-3NLS-CIB1-VP16-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL50
Plasmid#174068PurposeExpresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.DepositorInsertpTEF1-3NLS-97-3ZF-VP16
UseTagsFLAGExpressionYeastMutationPromoterpTEF1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL45
Plasmid#174067PurposeExpresses CIB1 in S. cerevisiae. Includes inducible lac operon.DepositorInsertpGal1-LacO-3NLS-CIB1-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
3919-32
Plasmid#176496PurposepCAG-puro-T2A-TetON3G-polyADepositorInsertpuroR-T2A-TetOn3G
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#174070PurposeExpresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal-Olac-3xNLS-VP16-CIB1-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL29
Plasmid#174063PurposeExpresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.DepositorInsert2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
UseTagsFLAGExpressionYeastMutationPromoterpCyc1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1211
Plasmid#129521PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFlag-NLSExpressionMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
scFv-IFP2.0N
Plasmid#215746PurposeExpresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0DepositorInsertIFP2.0N
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS:Zebrabow-B
Plasmid#118216PurposeFor broad gal4 inducible expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_empty_gRNA
Plasmid#197557Purposesites for cloning two gRNA with puromycin cassetteDepositorTypeEmpty backboneUseEmpty grna backbone with puromycin resistance geneTagsExpressionMammalianMutationPromoterU6 for gRNA, CMV for puromycin resistance.Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fPV-GFP
Plasmid#22914DepositorInsertfugu parvalbumin promoter driving GFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
ZJOM151
Plasmid#133643PurposeIntegration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.DepositorInsertp1k-COX4-GFP (COX4 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorInsertVph1-2GFP (VPH1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM31
Plasmid#133645PurposeIntegration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.DepositorInsertp1k-TGL3-GFP (TGL3 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-Sp dCas9-mVenusN
Plasmid#191396PurposeSpdCas9 fused to N-terminal part of mVenusDepositorInsertN-terminal part of split mVenus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1-14-mVenusC
Plasmid#191393PurposeMBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM18
Plasmid#133631PurposeIntegration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.DepositorInsertp1k-NAB2-GFP (NAB2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only