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Plasmid#53014PurposeAmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsfimS (off orientation)ExpressionBacterialMutationPromoterAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
IMS-APEX2
Plasmid#79058PurposeExpresses APEX in the mitochondrial intermembrane space.DepositorInsertAPEX
UseTagsV5ExpressionBacterial and MammalianMutationPromoterT7 promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_KD
Plasmid#79011PurposeFor mammalian expression of a kinase dead (K47R) version of AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationK47RPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 WT
Plasmid#16233DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 WT
Plasmid#16237DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Lact-C2
Plasmid#74061PurposeFluorescent biosensor for phosphatidylserine.DepositorInsertLactadherin-C2 (MFGE8 Bovine)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW62.YY1.FKBP.knock-in.mCherry
Plasmid#104370PurposeFor knocking in FKBP F36V P2A mCherry onto YY1DepositorInsertFKBP12(F36V) -P2A-mCherry
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorInsertSth1 Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail 6SA
Plasmid#16228DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutation6SA: S97A, S101A, S108A, S112A, S116A, S120APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-TRE-EGFP
Plasmid#89875PurposeEGFP reporter vector of iTango system for AAV productionDepositorInserttetO-EGFP
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCH67 (crRNA expression vector)
Plasmid#217337PurposeCROP-seq crRNA expression vector with GFP and puro markerDepositorInsertAsDR-filler-AsDR8, GFP-puro
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW63.YY1.FKBP.knock-in.BFP
Plasmid#104371PurposeFor knocking in FKBP F36V P2A BFPDepositorInsertFKBP12(F36V) -P2A-BFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-YFP-yeastSMASh
Plasmid#68855PurposeExpresses SMASh tag fused to YFP at C terminus in yeastDepositorInsertYFP-yeastSMASh
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-yeastSMASh-YFP
Plasmid#68854PurposeExpresses SMASh tag fused to YFP at N terminus in yeastDepositorInsertyeastSMASh-YFP
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only