Addgene

Showing: 2081 - 2100 of 13761 results

Results per page:

Sort results by:

pET28a-PTBP1-RRM12CCtoSS

Plasmid

#89155

Purpose

Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)

Use

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET - FnR062.Y78W

Plasmid

#84802

Purpose

bacterial expression of FnR.0.6.2-Y78W protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78W

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78W

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78D

Plasmid

#84786

Purpose

bacterial expression of FnR.0.6.2-Y78D protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78D

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78D

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78F

Plasmid

#84788

Purpose

bacterial expression of FnR.0.6.2-Y78F protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78F

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78F

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78G

Plasmid

#84789

Purpose

bacterial expression of FnR.0.6.2-Y78G protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78G

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78G

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78H

Plasmid

#84790

Purpose

bacterial expression of FnR.0.6.2-Y78H protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78H

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78H

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78I

Plasmid

#84791

Purpose

bacterial expression of FnR.0.6.2-Y78I protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78I

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78I

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78P

Plasmid

#84796

Purpose

bacterial expression of FnR.0.6.2-Y78P protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78P

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78P

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78R

Plasmid

#84798

Purpose

bacterial expression of FnR.0.6.2-Y78R protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78R

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78R

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78S

Plasmid

#84799

Purpose

bacterial expression of FnR.0.6.2-Y78S protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78S

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y78S

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78

Plasmid

#84783

Purpose

bacterial expression of FnR.0.6.2-Y78 protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pT7CFE1-TelR15-YPet

Plasmid

#49646

Purpose

TALEYellow-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

YPet

Expression

Mutation

Promoter

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Use

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PesaR-Plux

Plasmid

#47659

Purpose

PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-P

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Additional esa box downstream of the original esa…

Promoter

PesaR-P

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET28b+R3(R112C/T180C)

Plasmid

#108195

Purpose

Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C) fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET41s-GST-ATF6a-AD

Plasmid

#64774

Purpose

bacterial expression of GST-tagged ATF6 (1-150)

Depositor

Ronald Conaway, Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

ATF6a-AD (1-150) (ATF6 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

C-term deletion

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pETO18G-H7C/R86C

Plasmid

#27403

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Use

Tags

HisX8 and Thrombin

Expression

Bacterial

Mutation

Changes His 7 to Cys and Arg 86 to Cys

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)-SNAP-HCA_sensor

Plasmid

#127181

Purpose

Bacterial expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Use

Tags

HisTag and StrepTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only