Addgene

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pCS2-F1-GCG

Plasmid

#30699

Depositor

Scot Wolfe, Nathan Lawson

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCG

Use

Mrna production

Tags

HA tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GCT

Plasmid

#30700

Depositor

Scot Wolfe, Nathan Lawson

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCT

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
xfz8 probe/pBS

Plasmid

#16323

Depositor

Peter Klein

Article

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-8 (fzd8.S Frog)

Use

Tags

Expression

Bacterial

Mutation

Base pairs 1159-3240 of xfz8 cDNA

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pLIC.B4.TM1717

Plasmid

#11532

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2004 Sep 7. 10

Insert

YjeQ GDP binding protein (TM1717 T. maritima)

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam E2F3a

Plasmid

#37970

Depositor

Jacqueline Lees

Article

Lees et al Mol Cell Biol. 1993 Dec;13(12):7813-25.

Insert

E2F3a (E2F3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGTQL1221YC

Plasmid

#61705

Purpose

Gateway compatible BiFC vector (pEarleyGate202-YC)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-C and FLAG

Expression

Plant

Mutation

Promoter

35S

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Use

Tags

parS segments

Expression

Yeast

Mutation

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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NLRP3-mNeonGreen

Plasmid

#200942

Purpose

NLRP3 fused with mNeonGreen to indicate its localization change

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Expression

Mutation

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFG2

Plasmid

#87250

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Use

Tags

parS segments

Expression

Yeast

Mutation

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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Cdk6-HA

Plasmid

#1868

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D224Y

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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mPiezo1-IRES-eGFP

Plasmid

#80925

Purpose

expresses mouse Piezo1 with GFP

Depositor

Ardem Patapoutian

Article

Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

Insert

mouse Piezo1 CDS (Piezo1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk4-HA

Plasmid

#1876

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABEmax

Plasmid

#119815

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cdk2-DN-HA

Plasmid

#1885

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-TetOn-NLS-mCherry

Plasmid

#193762

Purpose

Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

NLS-mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mutation

SV40 NLS fused to mCherry

Promoter

TRE

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPV1exCellHalo

Plasmid

#220307

Purpose

Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

Depositor

Eric Senning

Article

Mott et al J Gen Physiol. 2024 (in press)

Insert

rTRPV1exCellHalo (Trpv1 Rat)

Use

Tags

circularly permutated Halotag (Deo et al., Nat Ch…

Expression

Mammalian

Mutation

linker included to insert cpHaloTag (

Promoter

CMV promoter

Available since

July 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(-)-mIFNa2

Plasmid

#135098

Purpose

Protein expression of mouse IFNa2 in mammalian cells

Depositor

Pablo Penaloza-MacMaster

Article

Palacio et al J Exp Med. 2020 Dec 7;217(12). pii: 1520

Insert

mIFN-α2 (Ifna2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pUltra

Plasmid

#24129

Purpose

3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

Depositor

Malcolm Moore

Article

Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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