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pMV-TC1_FR1

Plasmid

#217241

Purpose

Donor plasmid of TC1_FR1 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of TC1_FR1 transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-PARIS

Plasmid

#217244

Purpose

Donor plasmid of PARIS Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of PARIS transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-S_DM

Plasmid

#217249

Purpose

Donor plasmid of S_DM Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of S_DM transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-WzzE

Plasmid

#196661

Purpose

Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-WzzE

Plasmid

#196662

Purpose

Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

TEV-cleavable GST

Expression

Bacterial

Mutation

Valine insertion at position 2.

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-mCherry-WzzE

Plasmid

#196663

Purpose

Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Valine insertion at position 2 of WzzE.

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mCherry

Plasmid

#196664

Purpose

Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Use

Tags

TEV-cleavable distrep tag II and mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mClover3

Plasmid

#196665

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Use

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-GST-distrepII

Plasmid

#196666

Purpose

Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

GST and TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-distrepII

Plasmid

#196667

Purpose

Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-strep-mClover3

Plasmid

#196668

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

Plasmid

#214468

Purpose

Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

ZFYVE27 (ZFYVE27 Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S14D S25D S32D T261D

Promoter

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

Plasmid

#216694

Purpose

Synchronize trafficking of GPI-AP from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
SBP-mApple-FIREtag-GPI

Use

Tags

FIREtag, IL2 signal sequence, Streptavidin Bindin…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Use

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

Plasmid

#216533

Purpose

An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

Depositor

Yuki Kambe

Article

Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

Insert

axon-jGCaMP8m

Use

AAV and Cre/Lox

Tags

GAP43

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

Plasmid

#217704

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR6 (ADGRG6 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-840

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

Plasmid

#217737

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR6 (ADGRG6 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-846

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-EGFP-NEMO-D304N

Plasmid

#187898

Purpose

Mammalian cell expression of D304N mutation in NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

HA-EGFP-NEMO-D304N (Ikbkg Mouse)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

D304N

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

Plasmid

#214471

Purpose

Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

ZFYVE27 (ZFYVE27 Human)
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S14D S25D S32D T261D

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCR 2.1-TOPO Fzd6 N352A HA

Plasmid

#208365

Purpose

Extracellular HA-tagged Fzd6 N352A (mouse)

Depositor

Maureen Cetera, Danelle Devenport

Article

Cetera et al Development. 2023 Aug 25:dev.202078. doi

Insert

Fzd6 (Fzd6 Mouse)

Use

Cloning vector

Tags

HA

Expression

Mutation

Asparagine 352 to Alanine

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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