-
Plasmid#173865PurposeMammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)DepositorInsertmVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-L11
Plasmid#127831PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1-wt RhoA 3'UTR
Plasmid#26089DepositorInsertwt RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Skp2 promoter-luciferase
Plasmid#81119PurposeReporter activity of SKP2 gene promoterDepositorInsertSKP2 (Skp2 Mouse)
UseLuciferaseTagsluciferaseExpressionMutationcontains the region from 1_kb upstream of transcr…PromoterSKP2Available sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Vinculin-23
Plasmid#57978PurposeLocalization: Focal Adhesions, Excitation: 549, Emission: 565DepositorInsertVinculin (VCL Human)
UseTagsmOrange2ExpressionMammalianMutationV234L in vinculinPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB165 - pL1R3_pMAS::rLUC-I::tMAS
Plasmid#123198Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInsertpMAS::rLUC-I::tMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83A
Plasmid#58401PurposeExpresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 83 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24A
Plasmid#58400PurposeExpresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 24 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
5mCUC1 pGreenII0129
Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-PLtetO-1-UTR1-LacI-T500
Plasmid#45784DepositorInsertLac repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 sec
Plasmid#172062PurposeUAS vector to express GFP1-10 in the ER lumenDepositorInsertGFP1-10 sec
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6833
Plasmid#84983PurposeExpression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.DepositorInsertNudB-PMD
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-hygro-hPGAP3-3HA
Plasmid#50373PurposeExpress C-terminally triple HA tagged human PGAP3 in mammmalian cellsDepositorInsertPGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
UseTags3x HAExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-E2F-3'UTR-Mut
Plasmid#21171DepositorInsertE2F 3'UTR Mutant (E2F1 Human)
UseLuciferaseTagsExpressionMammalianMutation2 miR binding sites mutated. Changed from GCACTTT…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
kank2_pJet1.2
Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorInsertkank2 (LOC571549 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE28 XBC 40
Plasmid#16842DepositorInsertbeta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Flag-mMcl-1 KR
Plasmid#32983DepositorInsertMcl-1 (Mcl1 Mouse)
UseRetroviralTags3XFlagExpressionMammalianMutationAll Lys to ArgPromoterLTRAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorInsertERGIC2-WT (ERGIC2 Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab3 WT
Plasmid#37687DepositorInsertRab3 (Rab3 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE
Plasmid#107723PurposeFor expression of ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-103)
Plasmid#60357PurposeExpress the RRM1 of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-103) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 7-103 of Msi1PromoterT7Available sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk3a1(2nd methione)
Plasmid#15746DepositorInsertJnk3a1-2nd M (MAPK10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst1 SARAH
Plasmid#84297PurposeExpresses Mst1 SARAH domain for interaction studies.DepositorInsertMst1 (STK4 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7g2
Plasmid#14625DepositorInsertMKK7g2 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-RAP1
Plasmid#84525PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationno stop codonPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab10 DN
Plasmid#37709DepositorInsertRab10 (Rab10 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT23N (dominant negative)PromoterGAL4Available sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-35
Plasmid#63444PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertNN NI NN
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)
Plasmid#70194PurposeDerived from 21 with silent mutations to make BsaI, BbsI compatibleDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPPac-hp
Plasmid#48123PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterDepositorInsertsignal peptide of Pac
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1
Plasmid#129706PurposeExpress cre-dependent KASH-EGFP and Gmeb1 shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianMutationPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MMP2AG-T2APuroR-5h
Plasmid#128256PurposeMammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.DepositorInsertrtTA-Advanced::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPNprM-hp
Plasmid#48120PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoterDepositorInsertsignal peptide of NprM
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P30
Plasmid#120041PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-GDepositorInsertVSV-G
UsePcr templateTagsVSV-GExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hopscotch
Plasmid#37301DepositorInserthopscotch (hop Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab10 CA
Plasmid#37710DepositorInsertRab10 (Rab10 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ68L (constitutive active)PromoterGAL4Available sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Supervillin-45
Plasmid#55136PurposeLocalization: Membrane/Actin, Excitation: 587, Emission: 610DepositorInsertSupervillin
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S18
Plasmid#127827PurposeProduction of recombinant human ribosomal protein S18DepositorInsertRPS18 (RPS18 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
kank4_pJet1.2
Plasmid#121982Purposefor generating kank4 riboprobes for WISH in zebrafishDepositorInsertkank4 (kank4 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a2
Plasmid#14621DepositorInsertMKK7a2 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
SEPT11 sh2 shRNA
Plasmid#122023PurposeKnock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.DepositorInsertSEPT11 shRNA (Sept11 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Septin 11 isoform II-EGFP
Plasmid#120316PurposeSeptin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)DepositorInsertSEPT11 (Sept11 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-1
Plasmid#126056PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mAV
Plasmid#129472PurposeMammalian expression of full-length mouse archvillin (Supervillin, isoform 2)DepositorInsertSupervillin, isoform 2 (Svil Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP
Plasmid#114731PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab7 WT
Plasmid#37699DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only