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Plasmid#178489PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertTNKS_TANK-peptide-bind (TNKS Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOS003-lenti-CMV-GCaMPer
Plasmid#65227PurposeER calcium sensor for lentivirus productionDepositorInsertGCaMPer
UseLentiviralTagscircularly permuted GFPExpressionMammalianMutationGCaMP3 (D324G, D360G, D397G, D435G)PromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K107/113R-3xFlag_IRES-GFP
Plasmid#198392PurposeRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site double mutant (K107/113R)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-wild type mKdm2b-Flag
Plasmid#61739PurposeExpress wild-type mouse histone demethylase Kdm2bDepositorInsertKdm2b (Kdm2b Mouse)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX304 3xFlag-eRF1_IRES-GFP
Plasmid#198393PurposeeRF1 expressionDepositorInserteRF1 (ETF1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TAP DHX36
Plasmid#53547Purposemammalian expression of DHX36DepositorInsertDHX36 (DHX36 Human)
UseTagsTAPExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDG462
Plasmid#100903PurposeSpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorInsertLARP7 (LARP7 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
UseTags(-30)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-TVA-V5-RG
Plasmid#119743PurposeExpresses TVA, V5, and RG in a cre dependent manner.DepositorInsertTVA, V5, Rabies Glycoprotein
UseAAV and Cre/LoxTagsV5ExpressionMutationPromoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA14_G-alpha
Plasmid#109914PurposeProtein expression and purification of GNA14_G-alphaDepositorInsertGNA14_G-alpha (GNA14 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
UseTagslinked to red flouresent protein gene (katushka)ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GW-HA-RINT1
Plasmid#39841DepositorInsertRINT1 (RINT1 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromoterAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH4687
Plasmid#200783PurposePnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorinDepositorInsertins-22 (ins-22 Nematode)
UseTagspHiuorinExpressionWormMutationPromoterPnpr-4Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-P2A_BAF
Plasmid#101773PurposeExpresses BAF in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM190
Plasmid#69614Purposeinducible gene expression in StreptomycesDepositorInsertsaphII
tsr
rep
sso
TipA promoter
dso
UseStreptomyces- e. coli shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KPNA4_Arm
Plasmid#178495PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertKPNA4_Arm (KPNA4 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
011LP-pAAV-TNT4-EmGFP-pA
Plasmid#120280PurposeAAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoterDepositorInsertEmGFP
UseAAVTags3XFlag and HAExpressionMutationPromoterTNT Cardiac Troponin T promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct
Plasmid#86230PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA15-Ubc-NLS- scFV-tdTomato
Plasmid#199446PurposeExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cellsDepositorInsertscFV-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MAD2L1_HORMA
Plasmid#178480PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMAD2L1_HORMA (MAD2L1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD Mut
Plasmid#72218PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenusN-Cavin1
Plasmid#108887PurposeExpression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertcaveolae associated protein 1 (CAVIN1 Synthetic, Human)
UseTagsmVenus1-155ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsUseTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAG_14-3-3
Plasmid#109905PurposeProtein expression and purification of YWHAG_14-3-3DepositorInsertYWHAG_14-3-3 (YWHAG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-CoRESTfl
Plasmid#109156PurposeEncodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-HYG-YR
Plasmid#61765PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).DepositorInsertsHygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK S6K (E389-deltaCT) neo
Plasmid#58516PurposeLentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseLentiviralTagsHAExpressionMammalianMutationT389E, stop codon at amino acid 402PromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT81
Plasmid#218732PurposeExpresses N-terminally EGFP-tagged IFT81 in mammalian cellsDepositorInsertIFT81 (IFT81 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG_V5-PPP6C
Plasmid#92013PurposeExpresses V5-PPP6C, includes IRES-EmeraldDepositorInsertPPP6C (PPP6C Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-10
Plasmid#172743PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-10
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-mCherry-iLIDx6
Plasmid#103782Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-mCherry-iLIDx6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#92140PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
tph-1(short)::rpl-22-HA
Plasmid#122203PurposeC. elegans tagged ribosome expression plasmidDepositorInserttph-1(short)::rpl-22-HA
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTwt full-length
Plasmid#109161PurposeEncodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length REST/NRSF (REST Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only