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Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
UseTagsHisExpressionBacterialMutationWt genePromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3h
Plasmid#208699PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOS007: PercevalHR ATP/ADP sensor (cytosolic)
Plasmid#163061PurposePercevalHR ATP/ADP sensor (cytosolic)DepositorInsertPercevalHR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorInsertMB21D1 (CGAS Human)
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-ACTB-C1
Plasmid#169796PurposeExpresses Cas9 and guide RNA for ACTB gene in humanDepositorInsertguide RNA for ACTB gene
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHN922
Plasmid#178975PurposeExpression of GST-His8-GFP-nanobodyDepositorInsertGST-His8-GFP-nanobody
UseAffinity Reagent/ AntibodyTagsGST, His8ExpressionBacterialMutationPromotertacAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-C2
Plasmid#27339DepositorInsertSFFV promoter, mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG
Plasmid#169991PurposeMammalian purification construct for CARD8DepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hPAX7 (#348)
Plasmid#78337PurposeOverexpression of human PAX7 ORF with a N-terminal FLAG tagDepositorInsertPAX7 (PAX7 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-N1
Plasmid#54642PurposeLocalization: N1 Cloning Vector, Excitation: 554, Emission: 581DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA CO Tol2 TPase
Plasmid#133032PurposeExpression vector for production of codon optimized Tol2 transposaseDepositorInsertCodon optimized Tol2 Transposase
UseIn vitro expressionTagsExpressionMutationCodon OptimizedPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAG_GPHN.FingR-eGFP-CCR5TC
Plasmid#46296DepositorInsertGPHN.FingR-eGFP-CCR5TC (GPHN Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-CjCas9
Plasmid#89754PurposeExpression of CjCas9 with His tag in E.coliDepositorInsertCjCas9
UseCRISPRTags6XHis, HA, and SV40 NLSExpressionBacterialMutationPromoterT7Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHCglo
Plasmid#80169PurposeMinigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicingDepositorInsertsegments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Arp3-pmCherryC1
Plasmid#27682DepositorInsertArp3 (ACTR3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS-ACTB-C200-GFPfr1
Plasmid#169798PurposeTHe donor vector for the ACTB gene locus.DepositorInsertDonor sequence to ACTB gene for HR
UseHr donor vector for human actb gene.TagsExpressionMutationPartial sequence of GFP is inserted before the st…PromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorInsertADAR2 (ADARB1 Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-Q76L (CA)
Plasmid#83422PurposeMouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 76 from Q to L (Q76L: CAG -> CTG)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-mMegf10 CAR
Plasmid#113013Purposefor production of lentivirus encoding the CD19 CAR-Pmegf10DepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-ATP
Plasmid#193818PurposeCMV overexpression of ChemoG-ATP in mammalian cell linesDepositorInsertChemoG-ATP
UseTagsExpressionMammalianMutationEGFP:A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28HIS-hAPE1
Plasmid#70757Purposebacterial expression of human APE1DepositorInsertAPEX1 (APEX1 Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpot-Tag-GFP-GPI
Plasmid#117061PurposeFor mammalian expression of GPI-anchored GFP with N-terminal Spot-TagDepositorInsertGPI-anchored GFP
UseTagsGPI-anchor and Spot-TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec23A
Plasmid#66609PurposeGFP fusion of Sec23ADepositorInsertSec23A (SEC23A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
UseTagsExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Wt1
Plasmid#41082DepositorInsertWt1 (-KTS alternative isoform) (Wt1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetO + minimal CMVAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)
Plasmid#179115PurposeCargo EGFP with AttP Bxb1 site (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMPP-mCherry-mTrim21
Plasmid#104971PurposeLentiviral Transfer Plasmid with mCherry-tagged mouse Trim21DepositorInsertTripartite Motif containing 21 (Trim21 Mouse)
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTFG005_Spike_Inducible_3xFlag
Plasmid#191578PurposeInducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseLentiviralTags3x FLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX/PD-L1 WT
Plasmid#121466PurposeExpression of GST-human PD-L1 in E. ColiDepositorInsertCD274 (CD274 Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-hPAC-FLAG
Plasmid#129494PurposeExpresses human TMEM206 tagged at the C-terminus with FLAGDepositorInserthuman PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis
Plasmid#156948PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertNCAM1 (NCAM1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only