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pEGFP_L2509P-talin1 (1-2541)

Plasmid

#166123

Purpose

For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L2509P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L2509P

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_S1750F-talin1 (1-2541)

Plasmid

#166120

Purpose

For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

S1750F-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

S1750F

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2086V-talin1 (1-2541)

Plasmid

#166122

Purpose

For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

D2086V-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

D2086V

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_R1368W-talin1 (1-2541)

Plasmid

#166117

Purpose

For expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

R1368W-talin1-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

R1368W

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_Y1389C-talin1 (1-2541)

Plasmid

#166118

Purpose

For expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

Y1389C-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

Y1389C

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Article

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad2 (3A)

Plasmid

#101763

Purpose

Expresses SMAD2 (3A) in mammalian cells

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

SMAD2 (SMAD2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Three Ser to Ala mutations in the phosphorylation…

Promoter

CMV

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse Ism1 with C-terminal Myc-6X-his tag

Plasmid

#173046

Purpose

Expresses Ism1 in mammalian cells

Depositor

Katrin Svensson

Article

Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

Insert

Ism1 (Ism1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-scFVDnmt3AL_bGHpA

Plasmid

#177354

Purpose

AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)

Use

AAV

Tags

C-terminal domain of mouse Dnmt3l (194–415 aa), P…

Expression

Mammalian

Mutation

598–908 aa of mouse Dnmt3a

Promoter

Tetracycline-dependent promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKA-142

Plasmid

#79835

Purpose

Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

PpsR2-mVenus-CAAX

Use

Tags

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMVd1

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/SVNeo-103

Plasmid

#79841

Purpose

Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

RpBphP1-mCherry-Intersectin1 DHPH domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH)

Plasmid

#182368

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

glyceraldehyde-3-phosphate dehydrogenase (Gapdh Mouse)

Use

Tags

Pal-Dendra2

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV immediate-early enhancer/promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pSL3718 (pQCascade_Pse)

Plasmid

#200899

Purpose

Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCas7, PseCas8, PseCas6, PseTniQ

Use

Tags

NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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drebrin-YFP

Plasmid

#40359

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Chick expression

Tags

eYFP

Expression

Mutation

Promoter

chick B-actin promoter with CMV enhancer

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

Plasmid

#154826

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

mCherry
NeoR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Mutation

Promoter

CMV, EF1a, and SV40 early promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

Plasmid

#154827

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

TagBFP
HygR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Mutation

Promoter

CMV, EF1a, and SV40 early promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187916

Purpose

Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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