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pET28a-sfGFP

Plasmid

#85492

Purpose

pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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MP6

Plasmid

#69669

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGEN-luxCDABE

Plasmid

#44918

Depositor

Harry Mobley

Article

Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

sigma 70 promoter

Use

Luciferase

Tags

lux transcriptional fusion

Expression

Mutation

Promoter

em7

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4 HisMax-V5-GFP-RRBP1

Plasmid

#92150

Purpose

Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 (RRBP1 Human)

Use

Tags

Calreticulin signal peptide, EGFP (A207K mutation…

Expression

Mammalian

Mutation

In RRBP1: N951S and T1329L. E1279 is also missing.

Promoter

CMV

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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mKeima-Red-Mito-7

Plasmid

#56018

Purpose

Localization: Mitochondria, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Use

Tags

mKeima-Red

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIP-GFP-IRF3

Plasmid

#127663

Purpose

Over-express EGFP-IRF3

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

IRF3 (IRF3 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCXB-EBNA1

Plasmid

#41857

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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