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Plasmid#213779PurposeCloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.DepositorInsertSp gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2606
Plasmid#144050PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYRF (MYRF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)
Plasmid#160369PurposeStable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.DepositorInsertGPR143 (GPR143 Human)
UseLentiviralTagsmScarlet-3xHAExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1
Plasmid#213583PurposeDoxycycline inducible expression of YAP1 wild type cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScalps Luciferase Zsgreen
Plasmid#184784PurposeLentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoterDepositorInsertFirefly Luciferase and Zsgreen
UseLentiviral and LuciferaseTagsExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-FLAG
Plasmid#119233PurposePlasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.DepositorInsertTIE1 (TIE1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2072
Plasmid#141905PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYB (MYB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact EGFP
Plasmid#187686PurposeIn vivo visualization of actinDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-betaCatenin
Plasmid#114281PurposeTET-inducible expression of a constitutively active form of the BetaCatenin proteinDepositorInsertCatenin beta 1 (CTNNB1 Human)
UseLentiviralTagsFLAGExpressionMutationdeletion of AA 1 to 45PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-Nono-sh1
Plasmid#127650PurposeKnock-down of human NONODepositorInsertNONO shRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE53
Plasmid#90398PurposeSTAT1 - STAT1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT1Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_526
Plasmid#184859PurposeLentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1
Plasmid#125840Purposeexpress wild type BAP1DepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts2
Plasmid#115872PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts3
Plasmid#115873PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1892
Plasmid#144315PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertWNK1 (WNK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact-tdTomato
Plasmid#64048PurposeFluorescent reporter for F-actin labeling in living cellsDepositorInsertLifeact
UseLentiviralTagstdTomatoExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA SOX17 -177
Plasmid#50925PurposeU6 driven sgRNA targeting Sox17 -177 bp from TSSDepositorInsertSox17 -177 sgRNA (SOX17 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only