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Plasmid#59123PurposeFAK fret control sensor without FERM domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9G_arr2
Plasmid#149571Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9G(RBSmut)_arr2
Plasmid#149578Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX28
Plasmid#46850PurposeTOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiencyDepositorInsertattB, RFP and PGK-puro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K(-10TC)_arr2
Plasmid#149584Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K_arr2
Plasmid#149570Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K(RBSmut)_arr2
Plasmid#149577Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT
Plasmid#24606DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMYCExpressionMammalianMutationF29A, F30A, Y459APromoterAvailable sinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATF4 8: (SL-)5’ATF4.GFP
Plasmid#21855DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 KSR1 T274V
Plasmid#25975DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseTagsFlagExpressionMammalianMutationchanged threonine 274 to valinePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSO7
Plasmid#106365PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.DepositorInsertttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H08
Plasmid#70996PurposeGateway entry cloneDepositorInsertNap1 (Nap1 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN1
Plasmid#96979PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest53-SHP-1 S591D
Plasmid#21803DepositorInsertSHP-1 (PTPN6 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 591 to Aspartic acidPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 5
Plasmid#8479DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationG192DPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
Flag-NHR2 pcDNA3.1
Plasmid#17318DepositorInsertNeuralized-like (Neurl1a Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGSTag DLC2
Plasmid#24270DepositorInsertDLC2 (Dynll2 Mouse)
UseTagsGstExpressionBacterialMutationPromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
3441 pcDNA3 Bid G60A
Plasmid#8775DepositorInsertBid p22 G60A (Bid Mouse)
UseTagsExpressionMammalianMutationG60APromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) C716A
Plasmid#24607DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMYCExpressionMammalianMutationF29A, F30A, Y459A, C716APromoterAvailable sinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-Dest53-SHP-1 S591A
Plasmid#21802DepositorInsertSHP-1 (PTPN6 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 591 to AlaninePromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only