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Plasmid#113386PurposeExpress human alpha5 ectodomain with secretion peptide, purification tags and C-terminal claspDepositorInserthuman alpha 5 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT-mWasabi-G
Plasmid#164170PurposeExpresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backboneDepositorInsertmWasabi
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_E1770Q-talin1 (1-2541)
Plasmid#166121PurposeFor expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertE1770Q-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationE1770QPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron-G
Plasmid#36887DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-T
Plasmid#36885DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
UseTags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Puro/pTER shLUC (w589-1)
Plasmid#21472Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, PuroDepositorInsertFirefly luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1-EGFP
Plasmid#112857Purposeplasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion proteinDepositorInsertapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW2
Plasmid#166708Purposeexpresses NEDD4L with inactivating mutation in second WW domain in mammalian cellsDepositorInsertNEDD4L W453A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 453 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW3
Plasmid#166709Purposeexpresses NEDD4L with inactivating mutation in third WW domain in mammalian cellsDepositorInsertNEDD4L W525A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 525 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Blast/pTER shLUC (w618-1)
Plasmid#20962Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, BlastDepositorInsertFirefly luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo/pTER shLUC (w181-1)
Plasmid#17483Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, NeoDepositorInsertFirefly Luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UG3
Plasmid#133874PurposeExpresses UG3 in mammalian cells.DepositorInsertUtrCH (UTRN Human)
UseTagshelical linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Zeo/pTER shLUC (w648-1)
Plasmid#21561Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, ZeoDepositorInsertFirefly luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
RCAN1-aa1-150
Plasmid#65412PurposeRCAN1 aa1-150 expression with CMV promoter. Flag tagged.DepositorInsertRCAN1-aa1-150 (Rcan1 Mouse)
UseTagsFlagExpressionMammalianMutationaa1-150 present, deleted 151 to endPromoterAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT234_(pCA-G-intron-tTA2)
Plasmid#36874DepositorInsertsGFP
tTA2
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
Antares2-N1
Plasmid#120869PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertAkaluc
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW4
Plasmid#166710Purposeexpresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cellsDepositorInsertNEDD4L W576A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 576 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only