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Plasmid#170081PurposeGFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND
Plasmid#169963PurposepDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 Ub-S297
Plasmid#169965PurposepDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsUbExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 3'UTR GAC
Plasmid#110401PurposeLuminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporterDepositorInsertGLS glutaminase (GLS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-3'
Plasmid#110406PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2DepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
rs210-major
Plasmid#113377Purposeevaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)DepositorInsertrs2104047 major allele
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
rs210-minor
Plasmid#113378Purposeevaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)DepositorInsertrs2104047 minor allele
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR596Q-myc
Plasmid#107483PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationY289IPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTK605N-myc
Plasmid#107475PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K605N approxmately wildtype activity, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationK605NPromoterCMVAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTF465L-myc
Plasmid#107469PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F465L conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationF465LPromoterCMVAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR462E-myc
Plasmid#107489PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R462E with approximately wildtype activity, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationR462EPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG582K-myc
Plasmid#107497PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G582K conferring gain of function phenotype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG582KPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI576K-myc
Plasmid#107496PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576K with approximately wildtype activity, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationI576KPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTF556H-myc
Plasmid#107495PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F556H conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationF556HPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTP499K-myc
Plasmid#107494PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation P499K conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationP499KPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTE494Y-myc
Plasmid#107493PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E494Y conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationE494YPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTK490L-myc
Plasmid#107492PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K490L conferring gain of function phenotype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationK490LPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTL467D-myc
Plasmid#107490PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L467D with approximately wildtype activity, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationL467DPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTC473H-myc
Plasmid#107491PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation C473H conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationC473HPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTY410G-myc
Plasmid#107488PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y410G with approximately wildtype activity, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationY410GPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only