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AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189925

Purpose

AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Use

AAV

Tags

Expression

Mutation

SauriCas9 D15A

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-Inframe-stop

Plasmid

#177620

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Article

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Rep2

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Use

Tags

FIREmate

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-myc-CAR-2A-Thy1.1

Plasmid

#127890

Purpose

Expresses CART receptor along with tags.

Depositor

Anjana Rao

Article

Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

Inserts

myc,
CAR
2A
Thy1.1

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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CLXSN 5myc-CDC6-WT

Plasmid

#109332

Purpose

express tagged Cdc6 WT

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr-mKO-MK2

Plasmid

#115492

Purpose

Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

mKO-MK2 (MAPKAPK2 Human)

Use

Tags

monomeric Kusabira Orange (mKO)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Peroxi_C1

Plasmid

#189769

Purpose

Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Peroxi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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