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pPBbsr-mKO-MK2

Plasmid

#115492

Purpose

Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

mKO-MK2 (MAPKAPK2 Human)

Use

Tags

monomeric Kusabira Orange (mKO)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pOK12

Plasmid

#49789

Purpose

E. coli cloning vector (KanR, low copy, blue/white selection)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

lacZ

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSUMO1

Plasmid

#52258

Purpose

Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 )
SAE-1 (SAE1 Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest

Plasmid

#79784

Purpose

Expression plasmid - BsmBI entry sites

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-N 16E7

Plasmid

#37881

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16 E7 (E7 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
LVXN-Neo-NSD2

Plasmid

#86010

Purpose

express wild-type NSD2 in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 (NSD2 Human)

Use

Lentiviral

Tags

Flag tag D Y K D D D D K

Expression

Bacterial

Mutation

K1002R (please see depositor comments below)

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-XI-Neo-GST-Constitutively Active AMPK

Plasmid

#139843

Purpose

Lentiviral expression of GST-tagged Constitutively Active rat AMPK

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl3

Plasmid

#86680

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AXBEv2

Plasmid

#204606

Purpose

Mammalian expression of evolved adenine transversion base editor AXBEv2

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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