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pcDNA3 Flag beta-1-adrenergic-receptor

Plasmid

#14698

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B1AR (ADRB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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Lenti-LucOS

Plasmid

#22777

Depositor

Tyler Jacks

Insert

OVA-SIY

Use

Cre/Lox and Lentiviral

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-jRCaMP1b

Plasmid

#127873

Purpose

Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Red-shifted mitochondrial matrix Ca2+ indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Ddo_opsin

Plasmid

#107630

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

opsin

Use

RNAi; In situ hybridization

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pREDIA

Plasmid

#51625

Purpose

E. coli genome modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-nCas9

Plasmid

#163555

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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PH-Btk-mRuby2

Plasmid

#124657

Purpose

PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

BTK (BTK Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

contains only the PH domain of Bruton tyrosine ki…

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMH0006

Plasmid

#135448

Purpose

Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

Depositor

Jonathan Weissman, Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

dCas9-BFP-KRAB

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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