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pDN615

Plasmid

#175375

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN616

Plasmid

#175376

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN623

Plasmid

#175377

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN624

Plasmid

#175378

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN625

Plasmid

#175379

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN626

Plasmid

#175380

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-Sgd1

Plasmid

#100000

Purpose

Shuttle Sgd1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sgd1 (SGD1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Bms1

Plasmid

#100001

Purpose

Shuttle Bms1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Bms1 (BMS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb1

Plasmid

#99981

Purpose

Shuttle yeast Ckb1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb1 (CKB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb2

Plasmid

#99982

Purpose

Shuttle yeast Ckb2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb2 (CKB2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp11

Plasmid

#99984

Purpose

Shuttle yeast Utp11 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp11 (UTP11 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Pfa1

Plasmid

#99999

Purpose

Shuttle yeast Pfa1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Pfa1 (SQS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Cka1

Plasmid

#99979

Purpose

Shuttle yeast Cka1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka1 (CKA1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNJB99

Plasmid

#51521

Purpose

Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Zif268:FokI

Use

Gateway entry vector

Tags

NLS

Expression

Mutation

Promoter

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pYHY3

Plasmid

#216109

Purpose

aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

Depositor

Shannon Sirk

Article

Yeh et al bioRxiv. 2023 Dec 15:2023.12.14.571725.

Insert

NanoLuc

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

3xFLAG tag and BT3630 signal peptide

Expression

Bacterial

Mutation

Promoter

P1TDP-A21

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-sacB

Plasmid

#196249

Purpose

Plasmid containing the sacB gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attR2-sacB-attL3

Use

Tags

Expression

Bacterial

Mutation

Promoter

sacB promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Tags

Expression

Mutation

Promoter

Constitutive tracrRNA

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pYHY2

Plasmid

#216108

Purpose

Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

Depositor

Shannon Sirk

Article

Yeh et al bioRxiv. 2023 Dec 15:2023.12.14.571725.

Insert

NanoLuc

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

3xFLAG tag and BT0294 signal peptide

Expression

Bacterial

Mutation

Promoter

BfP1E6 promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCB591

Plasmid

#141096

Purpose

E. coli-Lactobacilli shuttle vector for recombineering template cloning

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJYDC1

Plasmid

#162458

Purpose

Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

HA tag, HDEL sequence, eUnaG2 fluorescent protein…

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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