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Plasmid#112560PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-AFV and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGEX4-4T-1 BCP1 NTase
Plasmid#161812PurposeExpresses SMODS/CD-NTase from Bacillus phage Bcp1DepositorInsertphage BCP1 SMODS/CD-NTase enzyme
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
6NIM
Plasmid#127294PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertPP-BRD18
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
5VS7 (PP-BRD4)
Plasmid#101264PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD4
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
5KO4 (PP-BRD19)
Plasmid#101882PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD19
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
5TCK (PP-BRD3)
Plasmid#101887PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
5U9N (PP-BRD3)
Plasmid#101255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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