Addgene

Showing: 2041 - 2060 of 49625 results

Results per page:

Sort results by:

SOX17-tdTomato-EhB

Plasmid

#210467

Purpose

Donor plasmid for knock-in tdTomato into the human SOX17 locus

Depositor

Kai Wang

Insert

SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SIRT6

Plasmid

#41564

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SIRT6 (SIRT6 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

aa 3-318

Promoter

T7

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits
IMBB2.4-pUC57-mini

Plasmid

#88845

Purpose

plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

Type

Empty backbone

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI-IRES-Bla-Ak-ACE2

Plasmid

#154981

Purpose

Lentiviral vector expressing Human ACE2

Depositor

Sonja Best

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
T-IL2-12

Plasmid

#166564

Purpose

scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, Sortag, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Q-IL18-6

Plasmid

#166554

Purpose

scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, Sortag, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV hSyn-Flex RG-cerulean

Plasmid

#98221

Purpose

Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus

Depositor

Troy Margrie

Insert

Cerulean-E2A-RG

Use

AAV

Tags

cerulean

Expression

Mutation

Promoter

human Synapsin

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-V5-DEST-hSFRP1-VF

Plasmid

#189542

Purpose

Protein expression of SFRP1 in mammalian cells.

Depositor

Haian Fu

Insert

secreted frizzled related protein 1 (SFRP1 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMB52 / Human_LRRK1_1_360

Plasmid

#40549

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK1 (LRRK1 Human)

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.2-V5-DEST-hSFRP1-his

Plasmid

#189540

Purpose

Protein expression of SFRP1 in mammalian cells.

Depositor

Haian Fu

Insert

secreted frizzled related protein 1 (SFRP1 Human)

Use

Tags

his

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-BE4max-P2A-EGFP (pRZ33)

Plasmid

#140083

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

Depositor

Keith Joung

Insert

Human codon optimized pCAG BE4max with P2A-EGFP

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

D10A (SpCas9)

Promoter

CAG

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV Agpat2-V5

Plasmid

#175159

Purpose

Lentiviral expression of mouse Agpat2-V5

Depositor

Larry Gerace

Insert

Agpat2 (Agpat2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV Cnep1r1-V5

Plasmid

#175133

Purpose

Lentiviral expression of mouse Cnep1r1-V5

Depositor

Larry Gerace

Insert

Cnep1r1 (Cnep1r1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SDCBPA-c000

Plasmid

#175480

Purpose

Protein expression in bacterial cells. Entire CDS, M1-V288.

Depositor

Opher Gileadi

Insert

SDCBP (SDCBP Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DAG1A-c003

Plasmid

#205107

Purpose

DAG1 protein expression

Depositor

Paul Brennan

Insert

DAG1 (DAG1 Human)

Use

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
G-BRD4-4

Plasmid

#166518

Purpose

scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2 PhyB (GB2721)

Plasmid

#160603

Purpose

chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.

Depositor

Diego Orzaez

Insert

PhyB

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Z-AIMP1-1

Plasmid

#166560

Purpose

scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
APEX2 VLP

Plasmid

#182211

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology; Tem imaging

Tags

Expression

Yeast

Mutation

Promoter

GAL1 and GAL10

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
integrin beta6 Y774F pBabe puro

Plasmid

#13599

Depositor

Dean Sheppard

Insert

integrin beta 6 Y774F (ITGB6 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Y774F

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only